Screening and Characterization of Soil Bacteria which Improves Crop Yield

  • N. Sai Prashanthi
Keywords: Organic Waste, Green Waste, Bacterial Consortium, Characterize Bacteria, Plant Growth, Promotion Activity


Municipal solid waste has 500 tons of waste per day with a composition of 60% of domestic waste and 40% of commercial waste. The composition of domestic waste were organic waste, green waste, mixed paper, plastic, textile, ferrous, glass, rubber and leather, and others. The degradation of organic wastes by the bacterial consortia is highly significant. This results in loss of potentially valuable materials that can be processed as fertilizer, fuel and fodder. The main aim of this project was to develop some successful bacterial consortium that can concomitantly degrade different components of the organic municipal solid wastes with the help of their enzymes in less span of time under natural conditions without producing any foul odor. In addition to that, the other important aim was to characterize the bacteria and their roles in plant growth promotion activity were observed in pot experiments.


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Abdul-Raouf UM, (1990). Studies of proteolytic bacteria isolated from certain localities in Aswan city. A thesisi (MSc.), Botany and microbiology Department, Faculty of Scince, Al-Azhar University, Cairo.

Acun˜a-Argu¨elles ME, Gutie´rrez-Rojas M, Viniegra-Gonza´lez G, Favela-Torres E, (1995). Production and properties of three pectinolytic activities produced by Aspergillus niger in submerged and solid-state fermentation. Appl Microbiol Biotechnol., 43: 808–814.

Adani F, Scatigna L, Genevini P, (2000). Biostabilization of mechanically separated municipal solid waste fraction, Waste Manage Res, 18: 471–477.

Adinarayana K, Ellaiah P, Prasad DS (2003) Purificationand Partial Characterization of Thermostable Serine Alkaline Protease from a Newly Isolated Bacillus subtilis PE-11. AAPS Pharm Sci Tech. 4: 1-9.

Ahmed I, Irfan1 M, Nadeem1 M, Zia MA, Ahmad BM Iqbal HMN (2010). Optimization of Media and Environmental Conditions for Alkaline Protease Production Using Bacillus Subtilis in Submerged Fermentation Process. IJAVMS. 4(4): 105-113

Agrawal D, Patidar P, Banerjee T, Patil S, (2004). Production of alkaline protease by Penicillum sp. under SSF conditions and its application to soy protein hydrolysis. Process Biochemistry, 39: 971-977.

Ahsan, N, (1999). Solid waste management plan for Indian megacities. Indian Journal Of Environmental Protection 19(2): 90–95.

Alexander, M. 1961 Introduction to Soil Microbiology, John Wiley and Sons, Inc, New York.

Alexander M, (1977). Introduction to soil microbiology. 2nd Edn. John Wiley and Sons Inc NEW YORK.

Alexander M, (1999). Biodegradation and Bioremediation. 2nd Ed.; Academic

Press: San Diego, USA, 325-327.

Allocati N, Federici L, Masulli M, Di Ilio C, (2009). Glutathione transferases in bacteria. The FEBS journal, 276(1): 58-75.

Ammar MS, (1975). Studies on Thermophilic Bacteria. Ph.D., Thesis, Botany

Department, Faculty of Science, Al-Azhar Universit y, Cairo, Egypt.

Ammar MS, Bayoumi RA, El-Kasaby AMH, Soliman AM, (2003). Purification and properties of thermostable protease by B. Brevis geltinoamylolyticus using fish wastes (Fi.W.) and poultry wastes (Po.W) under solid state fermentation (S.S.F.) conditions. 5th Int. Sic. Conf. Al-Azhar Univ. Fac. Sci. March 25-27, Cairo, Egypt. Pp.54.

Anesio, AM, Abreu PC, Biddanda BA, (2003). The role of free and attached microorganisms in the decomposition of estuarine macrophyte detritus. Estuarine, Coastal, and Shelf Science 56: 197–201.

Anwar A, Saleemuddin M, (1998). Alkaline proteases: a review.Bioresour Technol, 64: 175–183.

Aoyama M, Yasuda M, Nakachi K, Kobamoto N, Oku H, Kato F, (2000). Appl Microbiol Biotechnol. 53: 390-395.

APHA (American Public Health Association), (1992). Standard Methods for Examination of Water and Wastewater, Ed.18, American Public Health Association, Washington DC. ISBN: 0-87553-036-2.

Arantes V, Ferreira Milagres AM, (2007). The synergistic action of ligninolytic enzymes (MnP and Laccase) and Fe3+-reducing activity from white-rot fungi for degradation of Azure B. Enzyme and microbial technology, 42: 17-22.

AWPA (1986). American Wood Preservers Association Standards, Books of standards, Stevensville, Maryland 21666. USA.

Babu KR, Satyanarayana T, (1996). Production of bacterial enzymes by solid state fermentation. J Sci Ind Res. 55: 464–7.

Back EL, Salmen NL, (1982). Glass transitions of wood components hold implications for molding and pulping processes. Tappi., 65: 107-110.

Baladhandayutham S, Thagavelu V, (2010). Optimization and kinetics of pectinase enzyme using Aspergillus niger by solid-state fermentation. Ind.J. of Scien.and Technol., 3: 743-746.

Banerjee UC, Sani RK, Azmi W, Soni R. (1999). Thermostable alkaline protease from Bacillus bre6is and its characterization as a laundry detergent additive. Process Biochemistry, 35: 213–219.

Beg QK, Kapoor M, Mahajan L, Hoondal GS, (2001). Microbial xylanases and their industrial applications: a review. Appl. Microbiol. Biotechnol., 56: 326-338.

Beg QK, Sahai V, Gupta R, (2003). Statistical media optimization and alkaline protease production from Bacillus mojavensis in a bioreactor. Process Biochemistry, 39, 203.

Chykin S, (1966). Biochemistry laboratory techniques. John Wiley and Sons. New York, London, Sydney.

Clarridge JE, (2004). Impact of 16S rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases. Clin Microbiol Rev 17:840–862.

Colberg PJ, (1988). Anaerobic microbial degradation of cellulose, lignin, oligolignols, and monoaromatic lignin derivates. In Biology of Anaerobic Microorganisms. Zehnder, A. J. B. (ed). Wiley, USA., 333-372.

Collins CH, Lyne PM, (1987). Microbiological Methods, 5th ed., Butterworth & Co.Ltd., London.

Collins CH, Lyne PM, Grange JM, (1989). Microbiological Methods. 6th Ed. Butterworth. LONDON.

Cooper KE, (1355). Theory of antibiotic inhibition zones in agar media. Nature 176: 510-511.

Cowan DA, (1994). Industrial Enzymes, In Biotechnology-The science and the business, edited by V. Moses and R.E. Cape, (Harwood Academic Publishers, Switzerland, 326-328.

CPCB, 2000, Status of Solid Waste Generation, Collection, Treatment and

Disposal in Metrocities, Series: CUPS/46/1999–2000.

CPCB, 2000. Status of Municipal Solid waste Generation, Collection, Treatment and Disposal in Class I Cities, Series: ADSORBS/31/1999–2000.

Dalev PG, (1994). Utilisation of waste feathers from poultry slaughter for production of a protein concentrate Bioresource Technology, 48, 265.

Damodaran S, Han XQ, (1999). Detergent-stable alkaline protease from Bacillus pumilus, United States Patent Number 5976859.

Das G, Prasad MP, (2010). Isolation, purification & mass production of protease enzyme from Bacillus subtilis. Int Res J. microbial. 1(2): 026-031.

Dayal G, (1994). Solid wastes: sources, implications and management. Indian

Journal of Environmental Protection 14 (9): 669–677.

Debette J, (1991). Isolation and characterization of an extracellular protease produced by a soil strain of Xanthomonas maltophila. Curr Microbiol, 22:85–90.

Embley TM, Stackebrandt E. 1994. The molecular phylogency and systematic of the Actinomycetes. Ann Rev Microbiol 48: 257–289.

Erdogan R, Zaimoglu Z, Yavuz Sucu M, Fuat B, Secil K, (2008). Applicability of leachates originating from solid-waste landfills for irrigation in landfill restoration projects. J. Environ. Biol., 29: 779-784.

Evans R, Newman RH, Roick UC, Suckling ID, Wallis AFA, (1995). Changes in cellulose crystallinit y during kraft pulping-comparison of infrared, X-ray diffraction and solid-state NMR results. Holzforschung, 49: 498-504.

Felicita B, Nina H, MarijaV, Zoran G, (2003). Aerobic composting of tobacco industry solid waste-simulation of the process, Clean Technology Environmental Policy, 5: 295–301.

Ferrero MA, Castro GR, Abate CM, Biagori MD, Siňeriz F, (1996). Thermostable alkaline proteases of Bacillus licheniformis MIR 29: isolation, production and Characterization. Applied Microbiology and Biotechnology, 45, 327.

Findlay SEG, Dye S, Kuehn KA, (2002). Microbialgrowth and nitrogen retention in litter of Phragmites australis compared to Typha angustifolia. Wetlands, 22: 616–625.

Fogarty WM, Griffin PJ, Joyce AM, (1974). Enzymes ofBacillus species-Part 2.Proc Biochem. 9: 27–35.

Fujiwara N, Yamamoto K, (1987). Production of alkaline protease in a low-cost medium by alkalophilic Bacillus sp. and properties of the enzyme. Journal of Fermentation Technology, 65, 345.

Fujiwara N, Yamamoto K, Masui A, (1991). Utilization of a thermostable alkaline protease from an alkalophilic thermophile for the recovery of silver from used X-ray Film. Journal of Fermentation and Bioengineering, 72 (4): 306.

Gajju H, Bhalla TC Agarwal HO, (1996). Utilization of thermostable alkaline protease from Bacillus coagulans PB-77 for silver recovery from used X-ray films. In: Proceedings of 37th Annual Conference of Association Microbiologists of India, December 4–6, 1996, Chennai, India, (Abstract no. IM-4), pp. 79–79.

Gaub W H, Jr, (1924). A bacteriological study of a sewage disposal plant, N. J. Agri. Exp. Sta. Bull. Pp. 394.

Gautam SP, Bundela PS, Pandey AK, Awasthi MK, Sarsaiya S, (2010). Isolation, identification and cultural optimization of indigenous fungal isolates as a potential bioconversion agent of municipal solid waste, Annals of Environmental Science, vol. 5.

Gautam SP, Bundela PS, Pandey AK, Awasthi MK, Sarsaiya S, (2010). Optimization of the medium for the production of cellulase by the Trichoderma viride using submerged fermentation, International Journal of Environmental Science, 1(4): 656–665.

Gautam SP, Bundela PS, Pandey AK, Jamaluddin, Awasthi MK, Sarsaiya S, (2011). Diversity of CellulolyticMicrobes and the Biodegradation of Municipal SolidWaste by a Potential Strain. Hindawi Publishing Corporation International Journal of MicrobiologyVol. 2012, Article ID 325907, 12 pages doi:10.1155/2012/325907.

Gelsomino A, Keijzer-Wolters AC, Cacco G, Van Elsas JD, (1999). Assessment of bacterial community structure in soil by polymerase chain reaction and denaturing gradient gel electrophoresis. J. Microbiol. Methods, 38: 1-15.

Gessesse A, (1997). The use of nugmeal as a low cost substrate for the production of alkaline protese by the alkaliphilic Bacillus sp. AR 009 and some properties of enzyme. Bioresour. Technol. 62: 59-61.

Gillers RR, Govan JRW, (1966). Typical of Pseudomonas pyocyanea by pyocine production J . Path. Bact. 91, 33

Glawe U, Visvanathan C, Alamgir M, (2005). Solid waste management in least developed Asian countries - A Comparative Analysis International Conference on Integrated solid waste management in Southeast Asian cities, Siem Reap, Cambodia, pp. 5-7.

Kominkova D, Kuehn KA, Busing N, Steiner D, Gessner MO, (2000). Microbial biomass, growth, and respiration associated with submerged litter of Phragmites australis decomposing in a littoral reed stand of a large lake. Aquatic Microbial Ecology 22: 271–282.

Korsten L, Cook N, (1996). Optimizing culturing conditions for bacillus subtilis, South African Avocado Growers Association Yearbook, 19: 54-58.

Krishnamourthy RV, Vajranabhiah SN, (1986). Biological activities of earthworm casts. An assessment of plant growth promoter levels in the casts. Anim. Sci. 95: 341-351.

Kudrya VA, Simonenko IA, (1994). Alkaline serine proteinase and lectin isolation from the culture fluid of Bacillus subtilis. Applied Microbiology and Biotechnology, 41, 505.

Kuehn KA, Lemke MJ, Suberkropp K, Wetzel RG, (2000). Microbial biomass and production associated withdecaying leaf litter of the emergent macrophyte Juncuseffusus. Limnology and Oceanography 45: 862–870.

Kuhad RC, Singh A, Eriksson KEL, (1997). Microorganisms and enzymes involved in the degradation of plant fiber cell walls. In Advances in Biochemical Engineering/Biotechnology. Vol 57. Eriksson, K. E. L (ed). Springer, Germany., pp. 46-125.

Kumar CG, Malik RK, Tiwari MP, (1998). Novel enzyme-based detergents: An Indian perspective. Curr Sci 75: 1312–1318.

Kumar CG, Takagi H, (1999). Microbial alkaline proteases: from a bioindustrial viewpoint. Biotechnol Adv 17: 561–594.

Kumar R, Singh S, Singh OV, (2008). Bioconversion of lignocellulosic biomass: biochemical and molecular perspectives. Journal of industrial microbiology& biotechnology, 35(5): 377 391.

Kunamneni A, Camarero S, Garcia-Burgos C, Plou FJ, Ballesteros A, Alcalde M, (2008). Engineering and Applications of fungal laccases for organic synthesis. Microbial cell factories, 7:32.

Kuske CR, Barns SM, Busch J, (1997). Diverse uncultivated bacterial groups from soils of the arid southwestern United States that are present in many Geographic regions. Appl. Environ. Microbiol, 63(9): 3614-21.

Kwon YT, Kim JO, Moon SY, Lee HH, Rho HM, (1994). Extracellular alkaline protease from alkalophilic Vibrio metschnikovii strain RH530. Biotechnology Letters 16: 413–418.

Kyon YT, Kim JO, Moon SY, Lee HH, Rho, H. M, (1994). Extracellular alkaline proteases from alkalophilic Vibrio metschnikovii strain RH530. Biotechnol Lett 16: 413–418.

Laemmili UK, (1970). Cleavage of structural proteins during the assembly of the head of Bacteriophage T4. Nature. 277: 680-5.

LeBoeuf EJ, Weber WJ. Jr, (2000). Macromolecular characteristics of natural organic matter; Sorption and desorption behavior. Environ. Sci. Technol., 34: 3632-3640.

Lee CY, Cheng MF, Yu MS, Pan MJ, (2002). Purification and characterization of a putative virulence factor, serine protease, from Vibrio parahaemolyticus. FEMS Microbiol Lett. 19, 209 (1): 31-37.

Lee SY, (1996). Estimation of the abundance of an uncultured soil bacterial strain by a competitive quantitative PCR method. Appl. Environ. Microbiol., 62: 3787-93.

Lever M, (1973). Colorimetric and fluorometric carbohydrate determination with para-hydroxybenzoic acid hydrazide. Biochem. Med., 274-281.

Lichtfouse E, Chenu, C, Baudin F, (1996). Resistant ultralaminae in soils. Org. Geochem., 25: 263-265.

Lozano P, Manjo´n A, Romojaro F, Iborra JL, (1988). Properties of pectolytic enzymes covalently bound to nylon for apricot juice clarification. Process Biochem., 23, 75–78.

Mota FF, Gomes EA, Paiva E, Seldin L, (2005). Assessment of the biodiversity of Paenibacillus species in environmental samples by a novel rpoB-based PCR- DGGE method. FEMS Microbiol. Ecol., 53: 317-28.

Mullings, R, Parish JH, (1984). New reducing sugar assay for the study of cellulases. Enzyme Microb. Technol., 6: 491-496.

Myers RH, Khuri AI, Carter WH, (1989). Response surface methodology: 1966-1988. Technometrics, 15: 137-157.

Myers RH, Montgomery DC, (2002). Response Surface Methodology. John Wiley and Sons, USA.

Myers RH, Montgomery DC, Vining GG, Borror CM, Kowalski SM, (2004). Response surface methodology: A retrospective and literature survey. J. Qual. Technol., 36: 53-77.

Nakagawa A, (1994). Method for cleaning a contact lens. US Patent 5,314,823. Nakano MM, Zuber P, (1990). Molecular biology of antibiotic production in Bacillus. Crit. Rev. Biotechnol., 10(3): 223-40.

Odier E, Monties B, (1983). Absence of microbial mineralization of lignin in anaerobic enrichment cultures. Appl. Environ. Microbiol., 46: 661-665.

O’Shaughnessy FR, (1914). The utilization of the phenomena of putrefaction, with special reference to the treatment and disposal of sewage, J. Soc. Chem. Ind., 33: 3-9.

Sharholy M, Ahmad K, Mahmood G, Trivedi RC, (2005). Analysis of municipal solid waste management systems in Delhi – a review. In: Book of Proceedings for the second International Congress of Chemistry and Environment, Indore, India, pp. 773–777.

Shary S, Kapich AN, Panisko EA, Magnuson JK, Cullen D, Hammel KE, (2008). Differential expression in Phanerochaete chrysosporium of membraneassociated proteins relevant to lignin degradation. Appl Environ Microbiol., 74(23):7252-7257.

Shumi WM, Hossain T, Anwar MN, 2004. Proteolytic Activity of a Bacterial Isolate Bacillus fastidiosus den Dooren de Jong . JBS,. 4: 370-374.

Siddiqui TZ, Siddiqui FZ, Khan E, (2006). Sustainable development through integrated municipal solid waste management (MSWM) approach – a case study of Aligarh District. In: Proceedings of National Conference of Advanced in Mechanical Engineering (AIME- 2006), Jamia Millia Islamia, New Delhi, India, pp. 1168–1175.

Silver S, (1996). Bacterial resistances to toxic metals- a review. Gene 179: 9-19

Singh SK, Singh RS, (1998). A study on municipal solid waste and its management practices in Dhanbad–Jharia coalifield. Indian Journal of Environmental Protection 18 (11): 850–852.

Singh J, Vohra R.M, Sahoo D.K, 1999. Alkaline protease from a new obligate alkalophilic isolate of Bacillus sphaericus. Biotechnology Letters, 21, 921.

Singh J, Vohra RM, Sahoo DK., 1999. Alkaline protease from a new obligate alkalophilic isolate of Bacillus sphaericus. Biotech. Lett. 21: 921-924.

Singh SK, Singh RS, (1998). A study on municipal solid waste and its management practices in Dhanbad–Jharia coalifield, Indian Journal of Environmental Protection, 18(11): 850–852.

Sinsabaugh RL, Osgood MP, Findlay S, (1994). Enzymatic models for estimating decomposition rates of particulate detritus. Journal of the North American Benthological Society 13:160–169.

Sinsabaugh RL, Carreiro MM, Alvarez S, (2002). Enzyme and microbial dynamics during litter decomposition.Pages 249–266 in R. Burns and R. P. Dick, editors. Enzymesin the Environment. Marcel Dekker, New York, New York,USA

Smânia A, Monache FD, Smânia EFA, Cuneo RS, (1999). Antibacterial activity of steeroidal compounds isolated from Ganoderma applanatum (Pers.) Pat. (Aphyllophoro-mycetideae) Fruit body. Int. J. Med. Mushrooms, 1: 325-330.

Snedecor GW, Cochran WG, (1980). Statical methods. Lowa State Universit y Press, Ames, IA. Pp. 428

Wenzel M, Schonig I, Berchtold M, Kampfer P, Konig H, (2002). Aerobic and facultatively anaerobic cellulolytic bacteria from the gut of the termite Zootermopsis angusticollis. Journal of applied microbiology, 92(1): 32-40.

Whitman WB, Coleman DC, Wiebe WJ, (1998). Prokaryote the unseen majority. Proc. Natl Acad. Sci. USA, 95: 6578-83.

Wiley EO, Brooks DR, Siegel-Causey D, Funk VA, (1991). The Compleat Cladist: A Primer of Phylogenetic Procedures. Freely available at

William JB, Nicholas DS, Aaron LH, (2000). Weighted Neighbor Joining: A Likelihood-Based Approach to Distance-Based Phylogeny Reconstruction, Mol. Biol. Evol. 17 (1): 189-197.

Williams ST, Cross T, (1971.) Actinomycetes, methods in microbiology. vol 4, Academic Press, NEW YORK.

Wohl DL, McArthur JV, (2001). Aquatic actinomycete–fungal interactions and their effects on organic matterdecomposition: a microcosm study. Microbial Ecology, 42: 446–457.

Yossana S, Reungsangb A, Yasudac M, (2006). Purification and Characterization of Alkaline Protease from Bacillus megaterium Isolated from Thai Fish Sauce Fermentation Process, ScienceAsia 32: 377-383.

Zaldivar J, Nielsen J, Olsson L, (2001). Fuel ethanol production from lignocellulose: a challenge for metabolic engineering and process integration. Applied microbiology and biotechnology, 56(1-2): 17-34.

Zaved HK, Rahman MM, Rahman MM, Rahman A, Arafat SMY, Rahman MS, (2008). Isolation and Characterization of Effective Bacteria for Solid Waste Degradation for Organic Manure, KMITL Sci. Tech. J. pp. 844-855.

Zimmermann W, (1990). Degradation of lignin by bacteria. Journal of biotechnology.

How to Cite
N. Sai Prashanthi. (2020). Screening and Characterization of Soil Bacteria which Improves Crop Yield. International Journal for Research in Applied Sciences and Biotechnology, 7(4), 28-53.