Bio-Efficacy of Milastin-K (Bacillus subtilis KTSB 1015 1.5% A.S.) as a Potential Bio-Control Agent for Management of Bacterial Blight (Xanthomonas axonopodis) and Anthracnose (Colletotrichum gloeosporioides) Diseases in Pomegranate

  • Sandeepa Kanitkar
  • V. M. Raut
  • V. N. Shinde
  • T. B. Tambe
  • C. V. Ambadkar
  • Medha Kulkarni
  • Meghraj Kadam
Keywords: Pomegranate, Punica granatum, Milastin-K, Bacillus subtilis, Bacterial blight, Xanthomonas axonopodis, Anthracnose, Colletotrichum gloeosporioides, Bio-fungicide

Abstract

Field bio-efficacy study was conducted during 2018-19 for evaluation of Milastin-K (Bacillus subtilis KTSB 1015 1.5% A.S.) for control of bacterial blight (Xanthomonas axonopodis pv. punicae) and anthracnose (Colletotrichum gloeosporioides) on leaves, fruit and yield of pomegranate. The trial was conducted during Late Hasta Bahar at Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani. The trial was conducted in RBD with three replications and twelve treatments. Results compiled revealed that Milastin-K@ 2.0 ml/L each by spraying + soil drenching monthly at root zone recorded less bacterial blight disease severity (22.75 PDI each) on leaves and fruits with 61.20% and 54.16% disease control over untreated control (58.63 PDI and 49.63 PDI) respectively. Similarly, the same treatment recorded less PDI on leaves (36.81) and fruits (16.59) as compared to control (63.82 and 30.41) respectively with 43.56% and 45.45% disease control respectively for control of anthracnose disease on leaves and fruits giving 15.21 T/ha yield as compared to POP (11.71 T/ha) which is 29.88% more than POP  treatment. Similarly, there was no phytotoxicity in terms of leaf chlorosis, wilting, vein clearing, leaf tip burning, leaf necrosis and leaf epinasty.

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References

M. Abdel-Kader, M., S. El-Mougy, N., D. E. Aly, M., M. Lashin, S., & Abdel-Kareem, F. (2012). Greenhouse Biological Approach for Controlling Foliar Diseases of Some Vegetables. Advances in Life Sciences, 2(4), 98–103. https://doi.org/10.5923/j.als.20120204.03

Adinarayana, M. Narasegow, NC M. Anjanappa and V. Devappa (2018). Effect of bacillus subtilis var amyloliquefaciens on growth, yield and control of early blight in tomato. Int. J. Curr. Microbiol. App. Sci, Special Issue, 7, 656-664. Available at: https://www.ijcmas.com/special/7/Manukinda%20Adinarayana,%20et%20al.pdf

Ambadkar, C. V., Dhawan, A. S., & Shinde, V. N. (2015). Integrated management of bacterial blight disease (oily spot) of pomegranate caused by Xanthomonas axonopodis pv. Punicae. International Journal Plant Sciences, 10(1), 19–23.

Anonymous (2008). Studies on important disease of pomegranate. In Annual Report 2007-2008, N.R.C. on pomegranate, Solapur, pp. 24-26.

Apet K.T., R.C.Agale, O.S.Thakur and M.R.Tambe (2018). Efficacy of bio-agents and botanicals against Xanthomonas axonopodis pv.punicae causing bacterialblight of pomegranate. Int. J. Curr. Microbiol. App. Sci, Special Issue-6, 1905-1909. Available at: https://www.ijcmas.com/special/6/K.T.%20Apet2,%20et%20al.pdf

Atar, M. A. Efficacy of different antibiotics and botanicals for controlling bacterial blight [Xanthomonas axonopodis pv. punicae (Hingorani and Singh)] of pomegranate (Doctoral dissertation, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani).

Basamma, R.H. and Shripad Kulkarni. (2016). Bio-efficacy of bacillus subtilis against foliar fungal diseases of tomato. Intl. Jour App. Pure Sci. Agri. 2(2), 220-227.

Becatti, E., Chkaiban, L., Tonutti, P., Forcato, C., Bonghi, C., & Ranieri, A. M. (2010). Short-Term Postharvest Carbon Dioxide Treatments Induce Selective Molecular and Metabolic Changes in Grape Berries. Journal of Agricultural and Food Chemistry, 58(13), 8012–8020. https://doi.org/10.1021/jf100936x

Bettiol, W., Garibaldi, A., & Migheli, Q. (1997). Bacillus subtilis for the control of powdery mildew on cucumber and zucchini squash. Bragantia, 56(2), 281–287. https://doi.org/10.1590/S0006-87051997000200007

R. Chand and R. Kishun. (1991). Studies of bacterial blight (Xanthomonas campestris pv. punicae) of pomegranate. Indian Phytopath, 44(3), 370-372.

Chourasiya P. K., A. L. Abhilasha and S. Sabita (2013). Effects of certain fungicides and botanicals against early blight of tomato caused by alternaria solanii (ellis and martin) under allahabad, uttar pradesh india conditions. Int. Jour Agric. Sci. Res., 3(3), 151-156.

Desai S.G., M.K.Patel and M.V.Desai (1967) Invitro activity of streptocycline against bacterial plant pythogen. Indian Phytopath, 20(4), 296-300

S. Ferreira, J. H. (1991). Biological Control of Eutypa lata on Grapevine by an Antagonistic Strain of Bacillus subtilis. Phytopathology, 81(3), 283.

Gandhi, S.K. and Parashar, R.D.1978. Evaluation of some fungicides and antibiotics against Xanthomonas campestris causing bacterial rot of Raya. Indian Phytopathol, 31, 210-213.

Giri M. S., S. Prasanthi, K. S. Kulkarni. V. I. Benagi and Y. R. Hegade (2011). Biochemical and molecular variability among Xanthomonas axonopodis pv. punicae strains, the pathogen of pomegranate bacterial blight. Indian Phytopathol, 64(1), 1-4.

Hingorani, M. K. and P. P. Mehta. (1952). Bacterial leaf spot of pomegranate. Indian Phytopathol, 5, 55-56.

Hingorani M. K. and N. J. Singh. (1959). Xanthomonas punicae sp. Nov. on Punica granatum L. Indian Jour Agril. Sci., 29. 45-48.

Hooda, K.S.; U.C. Bhatt, D. Joshi and H.S. Gupta. (2008). Bio-control agents vis-a-vis. fungicides in managing various diseases of tomato (Lycopersicon esculentum Mill) in hills of Uttarkhand. Indian Phytopathol, 6(3), 331-336.

Hulloli, S.S.R.P. Singh, and J.P. Verma. (1998). Management of bacterial blight of cotton induced by Xanthomonas axonopodispv. malvacearum with use of neem based formulations. Indian Phytopathol, 512(1), 21-25.

Kanitkar Sandeepa, V.M. Raut, R. Achari and D.R. Patil. (2016). Fields evaluation of Milastin-K(Bacillus subtilis KTSB 1015 1.5% A.S.) as potential bio-control agent for powdery mildrew and downy mildew diseases in grapes. Pestology, 40(6), 21-31.

Kanitkar Sandeepa, V.M. Raut, S.D. Sawant, Medha Kulkarni and Meghraj Kadam. (2019). Bio-efficacy of Milastin-K(Bacillus subtilis KTSB-1015 1.5% A.S.) in controlling downy and powdery mildew disease in grapes. Pestology, 43(9), 27-39.

McKinay, H.H. (1923). A new system of grading diseases. Jour. Agril. Res., 26, 195-218.

Morsy Ebtasam M. (2005). Role of growth promoting substances producing micro organisms on tomato plant and control of some root rot fungi Ph.D. thesis. Fac. Agric. Ain Sham Univ, Cairo.

Mondal K.K. and D. Singh. (2009). Bacterial blight and emerging disease of pomegranate. In 5th International Conference on Plant pathology in the Glbalized Era. 10-13 November, 2009 I.A.R.I. New Delhi pp. 40.

Mulla, S. M., T.R. Mongale, V.D. Pillare and A.K.Pawar (2008). Studies on oily spot disease of pomegranate caused by Xanthomonas axonopodis pv. Punicae. Indian phytiopath, 63(3), 378.

Panse, V.G. and P.V. Sukhatme. (1985). Statistical Methods for Agricultural Workers, 2nd Edition. Indian Council of Agricultural Research, New Delhi pp.347.

Peterson, Y.M. E.L. Mansvelt, E. Venter and W.E. Langenhoven (2010). Detection of Xanthomonas axonopodis pv. punicae causing bacterial blight on pomergranate. Australian Pl. Path, 39, 544-546.

Premalatha Dath A. and Devadath, S. (1969). In vitro effect of antibiotics against Xanthomonas translucens sp.oryzae. Indian Phytopath, 22, 386-388.

Raju J., V.I. Benagi, K. Jayalakshms, V.B. Nargund and S.P. Sonavane. (2012). In vitro evaluation of chemicals, bactericals, and bio-agents against the bacterial blight of pomegranate caused by Xanthomonas axonopodis pv. punicae. Internation Journal Plant Protection, 5(2), 198-202.

Raghuwanshi K. S., B.A.Hujare, V.P.Chimoto andS.G.Borkar (2013). Characterizationof X.axonopodis pv punicae isolate from W. Maharashtra and their sensitivity to chemicals treatment. The Biscan, 8(3), 845-850.

Rangaswamy G. (1962). Pomegranate in Bacterial Plant Diseases in India. Asia Publication House, Bombay pp.830.

Rani U., K.S. Verma and K.K. Sharma. (2001). Pathogenic potential of Xanthomonas axonopodis pv. punicae and field response of different pomegranate cultivars. Plant Disease Research, 16, 198-202.

Ravikumar M.R., S.Y. Wali, V.I. Benagi, H.P. Patil and S.S. Patil (2011). Management of Bacterial blight of pomegranate through chemicals/ antibiotics Acta Horti 890. ISHS, 481-482.

Raut V.M., Sandeepa Kanitkar, Raghvendra Achari and D.R.Patil (2016). Field evaluation of Milastin-K(Bacillus subtilis KTSB 1015) a potential bio-control for powdery mildew and Downy Mildew diseases in grapes. Pestology, 40(6), 21-31.

Raut V.M., Sandeepa Kanitkar, Aditya Borawake, N.L.Meena and Virendra Singh (2017). Bio-efficacyof Milastin-K(Bacillus subtilis KTSB 1015) for Management of earlyblight disease of Tomato caused by Alternaria solani. Pestology, 41(1), 31-41.

Raut V.M., Sandeepa Kanitkar, Roopali Sharma and Aditya Borawake. (2018a). Effect of Bacillus subtilis KTYSB 1015 (Milastin-k) on growth yield and control of early blight disease in Tomato (Lycopersicon esculentum Mill). Pestology, 42(6), 15-24.

Raut V.M., Sandeepa Kanitkar, Roopali Sharma and Aditya Borawake. (2018b). Field evaluation of Milastin-K(Bacillus subtilis KTSB 1015 1.5% AS) as a potential plant growth promoting rhizobacteria in okra. Pestology, 42(7), 13-24.

Raut V.M., Sandeepa Kanitkar Narender Singh, Daljit Singh, Anita Arora and Aditya Borawake. (2018c) - Evaluation of Milastin-K(Bacillus subtilis KTSB 1015 1.5% AS) as a potential bio control agent for management of downy and powdery mildew in grape vines. Pestology 42(9), 15-24.

Raut V.M., Sandeepa Kanitkar, G.M.Manjunath, A.G.Nanjappanavar, Medha Kulkarni, and Meghraj Kadam (2019). Bio-efficacy of Sudo (Pseudomonas fluorescens) as a potential bio-control agent for management of bacterial blight (Xanthomonas qaxonopodis pv. punicae) and other foliar diseases in pomegranate. Pestology, 43(11), 23-33.

Sawant S.D., Indu S. Sawant, Dinesh Shetty, Manish Shinde, Sagar Jade and Manali Waghmare. (2011). Control of powdery mildew in vineyards by Milastin-k. A commercial formulation of Bacillus subtilis KTSB-1015. Jour Biological Control, 2(5), 26-32.

Schisler D.R., J.R. Slininger, R.W. Behle and M.S.Jackson. (2002). Formulation of Bacillus sp. for biological control of plant disease. Phytopathology, 1994, 1267-1276.

Sohi H.S., S.S. Jain, S.L. Sharma and B.R. Verma. (1964). New records for plant disease from H.P. Indian Phypatho, 17, 42-45.

Sharma R.R., Thind, B.S. and Singh, Nirmaljit (1979). In-vitro and In-vivo evulation ofchemicals against Xanthomonas vericatoria, the causal agent of bacterial leaf spot of chillies. Indian J. Mycol. & Pl. Pathol., 11(2), 178-182.

Vauterin L.B. Hoste, K. Kersters and J. Swings (1995). Reclassification of Xanthomonas. International Jour Systemic Bacteriology, 454, 472-489.

Published
2020-07-19
How to Cite
Sandeepa Kanitkar, V. M. Raut, V. N. Shinde, T. B. Tambe, C. V. Ambadkar, Medha Kulkarni, & Meghraj Kadam. (2020). Bio-Efficacy of Milastin-K (Bacillus subtilis KTSB 1015 1.5% A.S.) as a Potential Bio-Control Agent for Management of Bacterial Blight (Xanthomonas axonopodis) and Anthracnose (Colletotrichum gloeosporioides) Diseases in Pomegranate. International Journal for Research in Applied Sciences and Biotechnology, 7(4), 18-23. https://doi.org/10.31033/ijrasb.7.4.4
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Articles