Identification of an Antibacterial Potential Marine Streptomyces sps from Sargassum fluitans

  • Nitha N. Thomas
  • Archana V
  • Dr. Boby T. Edwin
Keywords: AIA (Actinomycetes Isolation Agar), ISP Medium (International Streptomyces Project), MHA (Muller Hinton Agar)


The discovery of new broad antibiotics is an urgent need to combatfrequently prompting diseases for social welfare. The marine environment posses most diverse environment from which novel secondary metabolites can be derived. The various number of bioactive compound has been identified from marine samples. To address this issue, there is chance for identifying bioactive compounds from marine actinomycetes. The aim of study was to isolate and identify secondary metabolites producing actinomycetes from marine algae (Sargassumfluitans.). The isolate SW A4 were partially characterised by morphological and biochemical methods. Their antibacterial activity werefurther analysed by preliminary and Disc diffusion assays. Interestingly, the SW A4 isolate showed excellent inhibition activity aganist certain disease causing human pathogenic bacteria. Here the methanolic extract showed efficient activity of 12,17,13,12 mm zoneaganist S.typhi, K.pneumonia, E.coli, B.cereus respectively.


Download data is not yet available.


Conover J. T. and J. M. Sieburth. (1964). Effect of sargassum distribution on its epibiota and antibacterial activity. Botanica Marina, 6(1/2), 147-157.

Edward J. Phlips, Marc Willis, and Anne Verchick. (1986). Aspects of nitrogen fixation in sargassum communities off the coast of florida. Journal of Experimental Marine biology and Ecology, 102(2–3), 99-119.

Anahit Penesyan, Zoe Marshall-Jones, Carola Holmstrom, Staffan Kjelleberg, and Suhelen Egan. (2009). Antimicrobial activity observed among cultured marine epiphytic bacteria reflects their potential as a source of new drugs. FEMS Microbiology Ecology, 69(1), 113–124. doi: 10.1111/j.1574-6941.2009.00688.x

Luis J. Villarreal-Gómez, Irma E. Soria-Mercado, Graciela Guerra-Rivas and Nahara E. Ayala-Sánchez. (2010). Antibacterial and anticancer activity of seaweeds and bacteria associated with their surface. Revista de Biologia Marina y Oceanograpfia, 45(2), 267-275.

Anurag P., and Anupam S. (2011). Utilization of carbon and nitrogen sources by streptomyces kanamyceticus m 27 for the production of an anti bacterialantibiotic. African Journal of Biotechnology, 4(9), 909-910.

Mounyr B, Moulay S, and Saad K. I. (2016). Methods for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical Analysis, 6(2), 71-79. doi: 10.1016/j.jpha.2015.11.005

E. B. Shirling and D. Gottlieb. (1966). Methods for characterization of Streptomyces species. International Journal of Systematic and Evolutionary Microbiology, 16(3), 313-340. doi: 10.1099/00207713-16-3-313

T. G. Pridham and David Gottlieb. (1948). The utilization of carbon compounds by some actinomycetales as an aid for species determination. Journal of Bacteriology, 56(1), 107-114.

L Selvameenal, M Radhakrishnan, R Balagurunathan. (2009). Antibiotic pigment from desert soil actinomycetes; biological activity, purification and chemical screening. Indian Journal for Pharmacological Science, 71(5), 499-504. doi:10.4103/0250-474X.58174

C. Dennis, and J. Webster. (1971). Antagonistic properties of species-groups of Trichoderma: III. Hyphal interaction. Transactions of the British Mycological Society, 57(3), 363-369.

R A Fromtling. (1988). Overview of medically important antifungal azole derivatives. Clinical Microbiological Reviews, 1(2), 187-217. doi: 10.1128/CMR.1.2.187

K. Nirmal Kumar, T. Ganesan and A. Elavarasi. (2013). Studies on antimicrobial activity of marine actinomycetes isolated from rameswaraminternational Journal of Pharmaceutical & Biological Archives, 4(4), 706 -710.

Saravana Kumar P, Duraipandiyan V, Ignacimuthu S. (2014). Isolation, screening and partial purification of antimicrobial antibiotics from soil Streptomyces sp. SCA 7. Kaohsiung J Med Sci, 30, 435–446.

Govindan Rajivgandhi, Govindan Ramachandran, Muthuchamy Maruthupandy, Subramaniyan Saravana kumar, Natesan Manoharan and Rajendran Viji. (2018). Antibacterial effect of endophytic actinomycetes from marine algae against multi drug resistant gram negative bacteria. Examines in Marine biology and Oceanography, 1(5), 132-139.

Mobeen Shaik, G. Girija Sankar, M. Iswarya, P. Rajitha. (2017). Isolation and characterization of bioactive metabolites producing marine Streptomyces parvulus strain sankarensis-A10. Journal of Genetic Engineering and Biotechnology, 15(1), 87-94.

How to Cite
Nitha N. Thomas, Archana V, & Dr. Boby T. Edwin. (2020). Identification of an Antibacterial Potential Marine Streptomyces sps from Sargassum fluitans. International Journal for Research in Applied Sciences and Biotechnology, 7(3), 53-58.