Synergistic Activity of Phenolic Compounds of Some Plants Against Bacteria

  • Roaa M. H. Shoker Department of Pharmacology, Collage of Medicine, Waist University, IRAQ
Keywords: Medicinal plants, Synergistic antibacterial effect, Phenolic extracts, HPLC analysis


Medicinal plants produce great groups of secondary metabolites which are essential for medicine purpose, one of them phenolic compounds, antimicrobial activity of phenolic compounds which derivative from plants has been examined for several years. The phenolic extracts of Sesamum indicum and Pimpinella anisum seeds have antibacterial action against Gram positive (Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli), (Acinetobacter baumannii), and (Pseudomonas aeruginosa) (Proteus mirabilis). The current findings show that the synergistic impact of phenolic extracts from S. indicum and P. anisum is active against a variety of pathogenic bacteria, and that the synergistic effect for two plants is more antibacterial than phenolic extracts from one plant.  The results indicated Gram- negative (P. aeruginosa) more effected by plants, than Gram-negative (S. aureus) which have the lower effects. The results of HPLC indicated Sesame (S. indicum) have total concentration of phenolic compounds was (1313.7 µg/ml) higher than total concentration of phenolic compounds of Anise (P. anisum) (220.991 µg/ml), and have varied types of phenolic compounds were Pyrogallol, Gallic acid, Rutin, Kaempferol, Cinnamaldehyde, Qurctin, Eugenol, Lignan with different concentration. From this study may be conclusion Synergistic effect for two plants have more antibacterial than phenolic extracts of one plant, and Sesame (S. indicum) have higher antimicrobial activity than Anise (P. anisum).


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Lambert, P.A. 2005. Bacterial resistance to antibiotics modified target sites Adv. Drug Rev., 57:1471-1485.

Sapkota, R.; R.N. Dasgupta, and D.S. Rawat. 2012. Antibacterial effect of plants extract on human microbial pathogens and microbial limit tests. Int J Res Pharm Chem., 2(4):2231-2781.

Thabit, A. K.; J. L. Crandon, and D. P. Nicolau. 2015. Antimicrobial resistance: impact on clinical and economic outcomes and the need for new antimicrobials. Expert Opin Pharmac other., 16: 159-177.

O'Neill, J. 2016. Tackling Drug-Resistant Infections Globally: Final Report and Recommendations. Review on Antimicrobial Resistance.

Schroeder, M.; B.D. Brooks, and A.E. Brooks. 2017. The complex relationship between virulence and antibiotic resistance. Genes (Basel), 8:39.

Rossiter, S. E.; M. H. Fletcher, and W. M. Wuest. 2017. Natural products as platforms to overcome antibiotic resistance. Chem Rev.,117: 12415-12474.

Saydut, A.; M. Z. Duz; C. Kaya; A. B. Kafadar, and C. Hamamei. 2008. Transesterified sesame (Sesamum indicum L.) seed oil as a biodiesel fuel. Bioresour Technol, 99:6656-6660.

Dravie, E. E.; N. K. Korteia; E. K. Essumana; C. O. Tettey; A. A. Boakye, and G. Hunkpe. 2020. Antioxidant, phytochemical and physicochemical properties of sesame seed (Sesamum indicum L). Scientific African 8.

Wu, M.-S.; L. B. B. Aquino; M. Y.U. Barbaza; C.-L. Hsieh; K. A. De Castro-Cruz; L.-L. Yang, and P.-W. Tsai. 2019. Anti-Inflammatory and Anticancer Properties of Bioactive Compounds from Sesamum indicum L.-A Review. Molecules, 24, 4426.

Al-Beitawi, N.A.; S.S. El-Ghousein, and H.N. Abdullah. 2009. Antibiotic growth promoters and anise seeds in broiler diets. Jordan Journal of Agricultural Sciences, 5(4):472-481.

Heywood, V. H.; R.K. Brummitt; A. Culham, and O. Seberg. 2007. Apiaceae In:

Flowering Plant Families of the World. New York, 35–38.

Sun, W.; M. H. Shahrajabian, and Qi Cheng. 2019. Anise (Pimpinella anisum L.), a dominant spice and traditional medicinal herb for both food and medicinal purposes. Cogent Biology, 5:1-25.

Harborne, J. B. 1984. Phytochemical methods. Chapman and Hall. New York 2nd ed. Pp: 288.

Mradu, G; S. Saumyakanti; M. Sohini; and M. Arup. 2012. HPLC profiles of standard phenolic compounds present in medicinal plants. IJPPR.,4 (3):162-167.

Nigam, D.; C. Singh, and U. Tiwari. 2014. Evaluation of in vitro study of antioxidant and antibacterial activities of methanolic seed extract of Sesamum indicum. Journal of Pharmacognosy and Phytochemistry, 3(4): XX-XX.

Henning, S.H.; A.Glick; D. Greenhalh; D. Morgan and S.Ysuopa. 2003. Catechin content of 18 teas and a green tea extracts supplements correlates with the antioxidant capacity nutrition and cancer, Cancer Biol. Ther.,45: 266-235.

Akinsulire, O.R.; E. A. Ibukun, A. Tayo; A. Toyin, and O. Tolu. 2007. In vitro antimicrobial activity of crude extracts from plants Bryophyllum Pinnatum and Kalanchoe Crenata. African Journal of Traditional, Complementary, and Alternative Medicines 4, 338-344.

Al-Bayati, F. A. 2008. Synergistic antibacterial activity between Thymus vulgaris and Pimpinella anisum essential oils and methanol extracts. Journal of Ethnopharmacology, vol. 116, no. 3, pp. 403–406.

Anilakumar, A. P.; F. Khanum, and A.S. Bawa. 2010.Nutritional, medicinal and industrial uses of sesame (Sesamum indicum L.) seeds-an overview. Agriculturae Conspectus Scientificus, 75 (4): 159-168.

Tabanca N.; E. Bedir; N. Kirimer; K.H. Baser; S.I. Khan; M.R. Jacob, and I.A. Khan. 2003. Antimicrobial compounds from Pimpinella species growing in Turkey. Planta Medical. 69:933-938.

Gulcin, I.; M. Oktay; E. Kirecci, and O. Irfan Kufrevioglu. 2003. Screening of antioxidant and antimicrobial activities of anise (Pimpinella anisum L.) seed extract. Food Chem., 83: 371-382.

Al-Kassie, G.A.M. 2008. The effect of anise and rosemary on the microbial babnce in gastrointestinal tract for broiler chicks. Int J Poult Sci., 7:610-612.

Chaudhry, N. M. and P. Tariq. 2006. Bactericidal activity of black pepper, bay leaf, aniseed and coriander against oral isolates. Pakistan Journal of Pharmaceutical Sciences, 19(3): 214-218.

How to Cite
Shoker, R. M. H. (2021). Synergistic Activity of Phenolic Compounds of Some Plants Against Bacteria. International Journal for Research in Applied Sciences and Biotechnology, 8(6), 102-110.