In Vitro Shoot Regeneration and Rooting of Piper Longum L.: A valuable Medicinal Plant
Piper longum L (Long pepper), of family Piperaceae, is an important medicinal plant in Sri Lanka. Cultivation of Piper longum L. till recently was not very common and still it is extensively collected from the wild owing to the increasing demand from the pharmaceutical industry, threatening the very existence of the plant. Poor seed viability and low germination, scanty and delayed rooting of vegetative cuttings restrict its propagation through sexual and vegetative propagation methods indicating a need of alternative approaches such as in vitro techniques for large scale propagation of this medicinally important plant. Therefore, this study was conducted to develop an effective in vitro propagation protocol for Long pepper using nodal segments as ex plants, obtained from a shade house grown mature plant. Murashige and Skoog (MS) medium was used throughout the experiment. For shoot proliferation, MS medium supplemented with combination of 1-Naphthalene acetic acid (NAA), Kinetin (KIN) and different levels of N6-benzylaminopurine (BAP) were used. In vitro rooting was achieved to 50% strength MS basal medium containing 1mg/L. The highest frequency of multiple shoot regeneration, that is 87.5% and the maximum number of, 36-40 shoots/ex plants in one sub culture (within 8 weeks after inoculation) were observed in MS media containing BAP (3 mg/L), KIN (0.5mg/L) and NAA (0.2mg/L). The maximum number of roots (6-8 per plantlet) were obtained in 50% MS basal medium + glucose (15 g/L) + Ascorbic acid (100 mg/ L) and gelled with 0.8% (w/v) agar supplemented with NAA (1 mg/L). In vitro rooted shoots were successfully acclimatized in the shade house conditions. Therefore, it is possible to deduce that the current protocol is promising for in vitro mass propagation of Piper longum L. to solve the reproduction and cultivation problem of the plant.
R. Parida, Y. Dhall, A study on the micro-propagation and antioxidant activity of Piper longum L. (an important medicinal plant). Journal of Medicinal Plants Research, 5: 6991-6994.2011.
V.V. Sivarajan, I. Balachandran, Ayurvedic drugs and their plant resources. Oxford and IBH Publishing Co Pvt. Ltd., USA, 1994.
A.M. Majumdar, J.N. Dhuley, V.K. Deshmukh, P.H. Raman and S.R. Naik, Anti-inflammatory activity of piperine, Japanese Journal of Medical Science and Biology, 43: 95-100, 1990.
B.S.Jung, M.K.Shin, Encyclopedia of illustrated Korean natural drugs, Araliaceae. In: Kwon SB (Ed) Encyclopedia of World Medicinal Plants. Seoul, 439-443, 1998.
K.K.N.Nair, Manual of Nonwood Forest Produce Plants of Kerala, Kerala Forest Research Institute, Kerala, India, 268-270, 2000.
V. Sarasan, V. Thomas, B. Lawrence, G.M. Nair, Plant regeneration in Piper longum L. (Piperaceae) through direct and indirect shoot development, Journal of Spices and Aromatic Crops 2: 34-40, 1993.
M. Phulwaria, K. Ram, K. Harish, A.K. Gupta and N.S. Shekhawat, .Micropropagation of mature Terminalia catappa (Indian almond),a medicinally important forest tree, Journal of Forest Research,17:202–207, 2012.
R. Parida and Y. Dhal, A study on the micro-propagation and antioxidant activity of Piper longum (an important medicinal plant), Journal of Medicinal Plants Research Vol. 5(32): 6991-6994, 2011.
M. Karim, R. Jannat, M. Rahman and M. Haque, Micropropagation of Stevia Plant from Nodal Segments. Progress. Agriculture, 19: 20-28, 2013.
E.V. Soniya, M.R. Das MR, In vitro micropropagation of Piper longum -an important medicinal plant. Plant Cell, Tissue and Organ Culture, 70: 325-327, 2002.
K. Sathelly, S. Podha, Establishment of Efficient Regeneration System from Leaf Discs in Long Pepper an Important Medicinal Plant (Piper longum L.), Med Aromat Plants 5:3, 2016.
G. Cristiano, B. Murillo-Amador and B. De Lucia, Propagation Techniques and Agronomic Requirements for the Cultivation of Barbados Aloe (Aloe vera (L.) Burm. F.)—A Review. Frontiers in Plant Science, 7, 2016.
E. Prakash, P.S. Sha Valli Khan, T.J.V. Sreenivasa Rao, E.S. Meru, Micropropagation of red sanders (Pterocarpus santalinus L.) using mature nodal explants. J.For. Res. 11, 329–335, 2006.
B. Rock-Okuyucu, M. Bayraktar, I. Akgun and A. Gurel, Plant Growth Regulator Effects on In vitro Propagation and Stevioside Production in Stevia rebaudiana Bertoni. Hort Science, 51(12): 1573-1580,2016.
Copyright (c) 2018 International Journal for Research in Applied Sciences and Biotechnology
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.