Paper Chromatographic Separation of Plant Pigments a New Approach by using Akshya-Swagatika Solvent and Mobile Chromatogram Detection System (MCDS)

  • Akshya Kumar Mishra School of Botany, Mahamaya Degree College, Nuapada, Odisha-766105, INDIA.
  • Swagatika Padhan School of Botany, Mahamaya Degree College, Nuapada, Odisha-766105, INDIA.
  • Arpita Behera School of Botany, Mahamaya Degree College, Nuapada, Odisha-766105, INDIA.
  • Madhusmita Naik School of Botany, Mahamaya Degree College, Nuapada, Odisha-766105, INDIA.
  • Jharana Nag School of Botany, Mahamaya Degree College, Nuapada, Odisha-766105, INDIA.
  • Rukmani Sahu School of Botany, Mahamaya Degree College, Nuapada, Odisha-766105, INDIA.
  • Hemant Raut School of Botany, Mahamaya Degree College, Nuapada, Odisha-766105, INDIA.
  • Gulshan Dandsena School of Botany, Mahamaya Degree College, Nuapada, Odisha-766105, INDIA.
  • Saudamini Karual School of Botany, Mahamaya Degree College, Nuapada, Odisha-766105, INDIA.
  • Rinki Harijan School of Botany, Mahamaya Degree College, Nuapada, Odisha-766105, INDIA.
  • Poonam Pradhan School of Botany, Mahamaya Degree College, Nuapada, Odisha-766105, INDIA.
  • R. Gowri Kalyani School of Botany, Mahamaya Degree College, Nuapada, Odisha-766105, INDIA.
  • Hira Bariha School of Botany, Mahamaya Degree College, Nuapada, Odisha-766105, INDIA.
  • Prangya Parimita Sahu School of Botany, Mahamaya Degree College, Nuapada, Odisha-766105, INDIA.
  • Balkishan Sahu School of Botany, Mahamaya Degree College, Nuapada, Odisha-766105, INDIA.
  • Sangita Jagat School of Botany, Mahamaya Degree College, Nuapada, Odisha-766105, INDIA.
  • Digesh Kumar Dandsena School of Botany, Mahamaya Degree College, Nuapada, Odisha-766105, INDIA.
  • Pranjalee Sharma School of Botany, Mahamaya Degree College, Nuapada, Odisha-766105, INDIA.
  • Duryodhan Pandey School of Botany, Mahamaya Degree College, Nuapada, Odisha-766105, INDIA.
  • Jagnyaseni Sahu School of Botany, Mahamaya Degree College, Nuapada, Odisha-766105, INDIA.
  • Ankita Sahu School of Botany, Mahamaya Degree College, Nuapada, Odisha-766105, INDIA.
  • Rajesh Ku. Sahu School of Botany, Mahamaya Degree College, Nuapada, Odisha-766105, INDIA.
  • Bhumika Sahu School of Botany, Mahamaya Degree College, Nuapada, Odisha-766105, INDIA.
  • Narendra Raut School of Botany, Mahamaya Degree College, Nuapada, Odisha-766105, INDIA.
  • Neetu Pradhan School of Botany, Mahamaya Degree College, Nuapada, Odisha-766105, INDIA.
  • Neha Sahu School of Botany, Mahamaya Degree College, Nuapada, Odisha-766105, INDIA.
  • Karuna Kar Pradhan School of Botany, Mahamaya Degree College, Nuapada, Odisha-766105, INDIA.
  • Bhomishma Chandrakar School of Botany, Mahamaya Degree College, Nuapada, Odisha-766105, INDIA.
  • Girija Sahu School of Botany, Mahamaya Degree College, Nuapada, Odisha-766105, INDIA.
Keywords: Plant pigments, Paper Chromatography, Akshya-Swagatika solvent, Mobile chromatogram detection system (MCDS)

Abstract

Chromatography is a term that refers to a group of laboratory techniques for separating mixtures. Chromatography works on the premise of solute partitioning between two phases or solvents. The technique of paper chromatography is commonly used to separate plant pigments based on their molecular weight. Plant pigments include chlorophyll-b, chlorophyll-a, carotenoid, and xanthophyll, which all have various molecular weights, colours, and absorption maxima. In this study, an attempt was made to see how a new solvent (Akshya-Swagatika solvent) can be used to separate plant pigments using paper chromatography, as well as a new detection method developed by us known as Mobile chromatogram detection system (MCDS) that can be used for compound identification and photographing. As shown in the table and photograph, pigments are separated by molecular weight and band colour, with carotenoid having the highest RF value and chlorophyll-b having the lowest. It was obvious that the Akshya-Swagatika solution could be used to separate plant pigments in paper chromatography. Photograph taken with a mobile chromatogram detection system that is much clearer than normal one. In developing countries, both solvent and detection systems are useful in explaining paper chromatography in a cost-effective manner. The MCDS detection system is cost-effective fast method which replaces traditional sophisticated detection procedures.

Downloads

Download data is not yet available.

References

Simon H Wender, Thomas B. Gaga (1949): paper chromatography of flavonoid pigments science 109(2829),287 -289 ,1949

EF Lind, HC Lane, LS Gleason (1953): partial separation of the plastid pigment by paper chromatography; Plant physiology 28(2),325-328,1953

Thomas Gaga, Carl Dougless, Simon Wender: identification of flavonoid compounds by filter paper chromatography analytical chemistry 23(11) 1582-1585 1951

Published
2021-11-02
How to Cite
Akshya Kumar Mishra, Swagatika Padhan, Arpita Behera, Madhusmita Naik, Jharana Nag, Rukmani Sahu, Hemant Raut, Gulshan Dandsena, Saudamini Karual, Rinki Harijan, Poonam Pradhan, R. Gowri Kalyani, Hira Bariha, Prangya Parimita Sahu, Balkishan Sahu, Sangita Jagat, Digesh Kumar Dandsena, Pranjalee Sharma, Duryodhan Pandey, Jagnyaseni Sahu, Ankita Sahu, Rajesh Ku. Sahu, Bhumika Sahu, Narendra Raut, Neetu Pradhan, Neha Sahu, Karuna Kar Pradhan, Bhomishma Chandrakar, & Girija Sahu. (2021). Paper Chromatographic Separation of Plant Pigments a New Approach by using Akshya-Swagatika Solvent and Mobile Chromatogram Detection System (MCDS). International Journal for Research in Applied Sciences and Biotechnology, 8(6), 1-3. https://doi.org/10.31033/ijrasb.8.6.1