Extraction of Lipopeptide from Bacillus subtilis and Histological Study on Albino Rats Tissue
Introduction and Aim: An extract of the lipopeptides from Bacillus subtilis has been found to be extremely useful in antimicrobial applications. Specifically, the goal of this study is to manufacture lipopeptide and assess the safety of this substance on the tissues and organs of laboratory rats.
Materials and Methods: Bacillus subtilis bacteria were isolated and identified at the postgraduate microbiology laboratory in the department of biology at Kufa University's College of Science. The bacteria became active after 24 hours of growth in the brain broth infusion broth medium. In order to determine the bacteria's capacity to produce lipopeptide. On nutrient agar medium, a quantity of lipopeptide was synthesized utilizing HCL for sediment, yielding an estimated amount of 1.5 gm during a two-month period, then the product was partially purified and lyophilized using a lyophilizer. For one week, the lipopeptide was administered on albino rat tissues such as the kidney, liver, spleen, and small intestine to determine its safety.
Results: The results showed no damage or any changes on tested tissues compared with control treatment and all of the previously mentioned organ tissues were completely intact and this is evidence of the safety of the lipopeptide extract for use.
Conclusions: It was discovered that lipopeptide had no effect on the organs that were utilized in the experiment and that it is safe for human consumption.
Banat IM, MakkardRS and Cameotra SS (2000) Potential commercial applications of microbial surfactants. Appl Microbiol Biotechnol.q53:495–508.
Coutte F, Lecouturier D, Dimitrov K, Guez JS, Delvigne F, Dhulster P, Jacques P (July 2017). "Microbial lipopeptide production and purification bioprocesses, current progress and future challenges" Biotechnology Journal. 12 (7): 1600566. doi:10.1002/biot.201600566.
Deleu M, Paquot M and Nylander T (2008) Effect of fengycin, ga lipopeptide produced by Bacillus subtilis, gon model biomembranes. Biophys J. 94:2667–2679f.
Fetoui H, Mahjoubi-Samet A, Jamoussi K, Ayadi F, Ellouze F, Zeghal N (2007) Nutr Res 27:788–793.
Grangemard., Bonmatin, J.M., Bernillon, J. Das. B.C. and Peyponx, F. (1999): Lichenysins G, a novel family of lipopeptide biosurfactants from Bacillus licheniformis. I M.1307: Production, Isolation and structural evaluation by MNR and mass spectrometry. J . Antibiot., 52, 363-373.
Hamley IW (May 2015). "Lipopeptides: from self-assembly to bioactivity" (PDF). Chemical Communications (Cambridge, England). 51 (41): 8574–83. doi:10.1039/ c5cc01535a.
Maneerat, S. and Phetrong, K. (2007). Isolation of biosurfactant-producing marine bacteria and characteristics of selected biosurfactant. Songklanakarin J. Sci. Technol., 29(3): 781-791.
Maniatis, T.; Fritsch, E. F. and Sambrook, J. (1982). Molecular cloning: A laboratory manual. Gold Spring Harbor Laboratory, New York.
Singh p. and Cameotra, s.s (2004): Potential applications of microbial surfactants in biomedical sciences. Trends Biotechnol.,22,142-146.
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