Role of Crisper CAS9 Technology in the Diagnosis of Covid19

  • Nishtha Chaturvedi Banasthali Vidyapith, Rajasthan, INDIA
  • Dr. Chhaya Chaturvedi Govt. Degree College, Laksar, Haridwar, Uttarakhand, INDIA
Keywords: Covid 19, pandemic, SARS-COV-2, diagnosis, RNA Guided Endonuclease (RGEN), RT PCR, CRISPER-Cas-9, antiviral therapy


Covid 19 is a pandemic disease came in existence in 2019. It is also called as the Human Corona Virus disease which is caused by SARS-CoV-2. Currently more than 215 countries around the world are being reported to be the sufferers of this disease. When this disease came into existence the diagnosis of the disease became a major task for the scientists and doctors across the world. A method known as RT PCR (Reverse Transcriptase Polymerase Chain Reaction) was applied during that crucial period to detect this disease. Among other diagnostic tools, CRISPER (Clustered Regularly Interspaced Short Palindromic Repeats) Cas system is being investigated for rapid and specific diagnosis of COVID-19. CRISPER-Cas technology is a highly flexible RNA guided endonuclease (RGEN) based nucleic acid editing tool that has transformed the field of genomics, gene editing, gene therapy and genome imaging. As compared to RT PCR which gives the result of diagnosis in 4-8 hours, the CRISPER Cas based method diagnose it within an hour. So, this technique came out to be a very appropriate and less time-consuming method for detection of the disease. This article is focuses on explaining the potential use of CRISPER Cas9 based approaches for the development of accurate and rapid diagnostic technique and antiviral therapy for detecting and limiting the spread of COVID-19. As this technique is new and advanced so can also be used for various other future applications too.


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Azhar M., Phutela R., Ansari A., Sinha D., Sharma N., Kumar M., et al. (2020). Rapid, field-deployable nucleobase detection and identification using FnCas9. Preprint. doi: 10.1101/2020.04.07.028167

Beloglazova N., Brown G., Zimmerman M. D., Proudfoot M., Makarova K. S., Kudritska M., et al. (2008). A novel family of sequence-specific endoribonucleases associated with the clustered regularly interspaced short palindromic repeats. J. Biol. Chem. 283, 20361–20371. doi: 10.1074/jbc.M803225200

Deltcheva E., Chylinski K., Sharma C. M., Gonzales K., Chao Y., Pirzada Z. A., et al. (2011). CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III. Nature 471, 602–607. doi: 10.1038/nature09886

Dhama K., Patel S. K., Pathak M., Yatoo M. I., Tiwari R., Malik Y. S., et al. (2020a). An Update on SARS-COV-2/COVID-19 with particular reference on its clinical pathology, pathogenesis, immunopathology and mitigation strategies–A Review. Travel. Med. Infect. Dis. 101755. doi: 10.1016/j.tmaid.2020.101755

Jackson R. N., van Erp P. B., Sternberg S. H., Wiedenheft B. (2017). Conformational regulation of CRISPR-associated nucleases. Curr. Opin. Microbiol. 37, 110–119. doi: 10.1016/j.mib.2017.05.010

Leroy E. M., Ar Gouilh M., Brugère-Picoux J. (2020). The risk of SARS-CoV-2 transmission to pets and other wild and domestic animals strongly mandates a one-health strategy to control the COVID-19 pandemic. One Health 13, 100133–100137. doi: 10.1016/j.onehlt.2020.100133,the%20molecular%20toolbox%20for%20biologists.&text=The%20CRISPR%E2%80%93Cas9%20system%20is,changes%20at%20defined%20target%20sites

Prashant Kumar, Yashpal Singh Malik, Balasubramanian Ganesh, Somnath Rahangdale, Sharad Saurabh, Senthilkumar Natesan, Ashish Srivastava, Khan Sharun Mohd Yatoo, Ruchi Tiwari, Raj Kumar Singh, Kuldeep Dhama Frontiers in cellular and infection microbiology 10, 639, 2020.

Roya Ramezankhani, Roya Solhi, Arash Memarnejadian, Fatemeharefeh Nami, Seyed MohammadReza Hashemian, Tine Tricot, Massoud Vosough, Catherine Verfaillie International Journal of Antimicrobial Agents, 106208, 2020

Tiwari R., Dhama K., Sharun K., Yatoo M. I., Malik Y. S., Singh R., et al. (2020). COVID-19: animals, veterinary and zoonotic links. Vet. Q. 40, 169–182. doi: 10.1080/01652176.2020.1766725

Zhang J., Kasciukovic T., White M. F. (2012). The CRISPR associated protein Cas4 Is a 5′ to 3′ DNA exonuclease with an iron-sulfur cluster. PloS One 7, e47232. doi: 10.1371/journal.pone.0047232

Zuris J. A., Thompson D. B., Shu Y., Guilinger J. P., Bessen J. L., Hu J. H., et al. (2015). Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo. Nat. Biotechnol. 33, 7

How to Cite
Nishtha Chaturvedi, & Chhaya Chaturvedi. (2021). Role of Crisper CAS9 Technology in the Diagnosis of Covid19. International Journal for Research in Applied Sciences and Biotechnology, 8(5), 162-167.