Effect of IBA, NAA and their Combination on Rooting and Biochemical Parameters of Stem Cuttings in Barbados Cherry

  • Alam Khan Samim Agriculture Faculty, Paktia University, AFGHANISTAN.
  • B. S. Shivakumar College of Horticulture Mudigere- 577132, University of Agricultural and Horticultural Sciences, Shivamogga, Karnataka, INDIA.
  • Ganapathi M. College of Horticulture Mudigere-577132, University of Agricultural and Horticultural Sciences, Shivamogga, Karnataka, INDIA.
Keywords: Barbados cherry, rooting, biochemical parameters, stem cutting, plant growth regulators


An investigation was to assess the influence of growth regulators IBA, NAA and their combination on rooting and biochemical parameters of stem cuttings in Barbados cherry. It was observed that, the least number of days (21.33) taken for root initiation, maximum percentage of rooting (80.00) was observed in cutting treated with IBA 5000 ppm followed by IBA 4500 ppm over other treatments. Significantly highest Chlorophyll-a (1.26), Chlorophyll –b (0.55) and total chlorophyll content (1.82 mg/g of fresh weight) of leaves and C : N ratio (8.74) was observed in cuttings treated with IBA 5000 ppm followed by IBA 4500 ppm over all other treatments.


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Abdulqader SM, Abdulrhman AS, Ramazan. ZI. Effect of wounding and different concentration of IBA on the rooting and vegetative growth of stem cutting of three olive cultivars. Kufa J. Agri. 2017; 2(9): 203-225.

AKSHAY, K. R., 2012, Effect of media and growth regulators on rooting of black pepper (Piper nigrum L.) cuttings. M.Sc. Thesis, Univ Hort. Sci., Bagalkot.

BASU, R. N. AND GHOSH, S. K., 1974, Effects of nitrogen nutrition of stock plants of Justica gendarussa L. on the rooting of cuttings. Hort Sci., 49: 245-252.

Chandramouli H. Influence of growth regulators on the rooting of different types of cuttings in Bursera penicilliatai (DC) Engl. M.Sc. Thesis, Univ. Agric. Sci. Bangalore, 2001.

DUSMAN, E., FERREIRA, M.F., BERTI, A.P., MARIUCCI, R. G. AND MANTOVANI, M. P., 2012, Investigation of cytotoxic and mutagenic effects of Malpighia glabra L. (Barbados cherry) fruit pulp and vitamin C on plant and animal test systems. Ciência e Tecnologia de Alimentos, 32(2): 405-411.

GHATNATTI, S. A., 1997, Studies on propagation of Duranta plumeri Jacq. var. Goldiana by stem cuttings with growth regulators under mist. M.Sc. Agri. Thesis, Univ, Agric. Sci., Dharwad.

HEDGE, J. E. AND HOFREITER, B. T., 1962, In: Carbohydrate chemistry, Ed., Academic Press, New York.

KAUNDAL, G. S. AND SINGH, M., 1989, IBA induced rhizogenesis and periodic metabolic changes in pear. J. Res, Punjab Agric. Univ., 26: 47-50.

KAUR, S., CHEEMA, S. S., CHHABRA, B. R. AND TALWAR, K. K., 2002, Chemical induction of physiological changes during adventitious root formation and bud break in grapevine cuttings. Plant Growth Regul., 37: 63-68.

MOKASHI, A.N., 1978, Studies on propagation of ‘Thompson Seedless’ grape (Vitis vinifera L.) by cuttings. Mysore J. Agric. Sci., 12(5): 5-8.

A.O. A. C., 1970, Official methods of analysis in the association of official analytical chemists, 11th Ed., Washington.

PRASAD, T. K., MEHTA, F., DAVE, Y. S. AND SUMA, T. K., 1980, Biochemical changes on regenerating root cutting of Clerodendron infortunatum. Indian J. Exptl. Biol., 18: 1524-1525.

RAO, M. B. N., SATYANARAYANA, G., RAMESHWAR, A., RAJ, A. E., GNANAKUMAR, N. AND PADMANABHAV., 1988, Interaction effect of certain phenolic compounds with IBA on induction of rooting in stem cutting of cashew. Ind. Cashew J., 18: 9-10.

Shivaji T, Madhavi K, Sudhavani V. Effect of type of cuttings and IBA concentrations on the propagation of Fig (Ficus carica) cv. Poona Fig under open conditions. Trends of Biosciences. 2014; 7(11): 1087-1090.

Singh KK. Effect of IBA concentrations on the rooting of pomegranate (Punica granatum L.) cv. Ganesh hardwood cuttings under mist house condition. International. J Horti. and Flori. 2017; 5(4): 318-323.

SINGH, K. K., CHOUDHARY, T. AND KUMAR, A., 2014, Effect of various concentration of IBA and NAA on the rooting of stem cuttings of mulberry (Morus alba L.) under mist house condition in Garhwal hill region. Indian J. Hill Farming., 27(1): 125-131.

Caula. A. B., and Robert. N. T. (2008) Plant Propagation Concept and Laboratory Exercises. CRC Press, pp.394

How to Cite
Alam Khan Samim, B. S. Shivakumar, & Ganapathi M. (2021). Effect of IBA, NAA and their Combination on Rooting and Biochemical Parameters of Stem Cuttings in Barbados Cherry. International Journal for Research in Applied Sciences and Biotechnology, 8(5), 147-150. https://doi.org/10.31033/ijrasb.8.5.21