Estimation of IL-6 and IL-10 Levels in Patients with Iron Deficiency Anemia

  • Rabaa Khaled Abdel Salam College of Science, Tikrit University, IRAQ.
  • Saria Naji Mohsin College of Science, Tikrit University, IRAQ.
Keywords: iron deficiency anemia, hepcidin, IL-10, IL-6

Abstract

The study included a process of diagnosing and differentiating (60) cases of iron deficiency anemia patients (30) moderate iron deficiency anemia and (30) severe iron deficiency anemia, who are residents of the city of Kirkuk and its suburbs, and their ages ranged from 18-30 years of both sexes (30 males). And 30 females (with a control group of healthy (30) of whom (15 males and 15 females) and their ages ranged from 18-30 years. Anemia was diagnosed by following several basic measures such as the total blood picture count, which included the compacted cell volume (PCV), hemoglobin level, the RBCs red blood cell count, as well as the corpuscular hemoglobin rate (MCH) and the corpuscular volume rate (MCV) in addition to Other discriminatory measures such as serum iron and ferritin Serum Hepcidin, a marker of iron deficiency anemia, was significantly lower in the case of IDA patients. It has been found that IL-6 plays an important role in hepcidin regulation particularly in IDA. In the case of IDA, the level of IL-6 was significantly decreased. And we observed elevated IL-10 in IDA patients. We found that IL-10 has a role in hepcidin inhibition through IL-6. Inhibition.

Downloads

Download data is not yet available.

References

Mohamed, M. S. A. (2019). Evaluation of Plasma Interleukin-10 Level in Sudanese Iron Deficiency Anemia Patients among Khartoum State (Doctoral dissertation, Sudan University of Science and Technology).‏

Nemeth, E.; Tuttle, M.S.; Powelson, J.; Vaughn, M.B.; Donovan, A.; Ward, D.M.; Ganz, T.; and Kaplan, J. (2004). Hepcidin regulates cellular iron efflux by binding to ferroportin and inducing its internalization. Science, 306, 2090–2093.

Beard, J.L. (2001). Iron Biology in Immune Function, Muscle Metabolism and Neuronal Functioning. The Journal of nutrion, 4(3):568-579.

Chandra RK. Nutrition and the immune system from birth to old age. Eur J Clin Nutr 2002; 56: 73–76.

Beard, J.L. (2001). Iron Biology in Immune Function, Muscle Metabolism and Neuronal Functioning. The Journal of nutrion, 4(3):568-579.

Salman AH, Iqbal MN, Bakir WA. The In Situ Expression of IL-6and IL-1β in breast cancer patients. Iraqi J Pharm Sci. 2008 Nov; 17 (2):55 – 62.

Tripsianis G, Papadopoulou E, Romanidis K, Katotomichelakis M, Anagnostopoulos K, Kontomanolis E, et al. Overall Survival and Clinicopathological Characteristics of Patients with Breast Cancer in Relation to the Expression Pattern of HER-2, IL 6, TNF-α and TGF-β1. Asian Pac J Cancer Prev. 2013; 14(11): 6813 –6820. Available from http:// dx.doi.org DOI: 10.7314/APJCP.2013.14.11.6813 infectious disease. J Nutr 2001; 131: 6168–6355.

Fuster, J.J. and Walsh, K., 2014. The Good, the Bad, and the Ugly of interleukin‐6 signaling. The EMBO journal, p.e201488856.

Wang, Y., van Boxel-Dezaire, A.H., Cheon, H., Yang, J. and Stark, G.R., 2013. STAT3 activation in response to IL-6 is prolonged by the binding of IL-6 receptor to EGF receptor. Proceedings of the National Academy of Sciences, 110:16975-16980.

Grossman, R.M., Krueger, J., Yourish, D., Granelli-Piperno, A., Murphy, D.P., May, L.T., Kupper, T.S., Sehgal, P.B. and Gottlieb, A.B., 1989. Interleukin 6 is expressed in high levels in psoriatic skin and stimulates proliferation of cultured human keratinocytes. Proceedings of the National Academy of Sciences, 86:6367-6371

Sheikhpour E, Noorbakhsh P, Foroughi E, Farahnak S, Nasiri R, Neamatzadeh H. A Survey on the Role of Interleukin-10 in Breast Cancer: A Narrative. Rep Biochem Mol Biol. 2018 Jul; 7 (1): 30 – 37

Sung W – W, Lee H. The role of interleukin-10 in the progression of human papilloma virus - associated lung carcinoma. Onco Immunology .2013 Jul [2013]; 2 (9): e25854-1 - e25854-3. Available from http:// dx.doi.org DOI: 10.4161/once.25854

Huang, P., Wang, J., Lin, X., Yang, F.F., Tan, J.H. (2017). Effects of IL-10 on iron metabolism in LPS-induced inflammatory mice via modulating hepcidin expression. European Review of Medicine and Pharmacological Sciences, 21(15):3469-34

Tian K, Zhang R, Wang X. Association of interleukin-10 polymorphisms and haplotypes with the risk of breast cancer in northern China. Int J Clin Exp Pathol. 2017 Apr; 10(6):6989 – 699

Emiralioglu, N., Yenicesu, I., Sari, S., Egritas, O., Poyraz, A., Pasaoglu, O. T., . . . Dalgic, B. (2015). An insight into the relationships between prohepcidin, iron deficiency anemia, and interleukin-6 values in pediatric Helicobacter pylori gastritis. European journal of pediatrics, 174(7), 903-910.

Hassan, T. H., Badr, M. A., Karam, N. A., Zkaria, M., El Saadany, H. F., Rahman, D. M. A., ... & Selim, A. M. (2016). Impact of iron deficiency anemia on the function of the immune system in children. Medicine, 95(47)

Ekiz, C., Agaoglu, L., Karakas, Z., Gurel, N., & Yalcin, I. (2005). The effect of iron deficiency anemia on the function of the immune system. The Hematology Journal, 5(7), 579-583.

Nakagawa, H., Tamura, T., Mitsuda, Y., Goto, Y., Kamiya, Y., Kondo, T., . . . Hamajima, N. (2014). Inverse correlation between serum interleukin-6 and iron levels among Japanese adults: a cross-sectional study. BMC hematology, 14(1), 6-6. doi: 10.1186/2052-1839-1

Akchurin, O., Patino, E., Dalal, V., Meza, K., Bhatia, D., Brovender, S., . . . Kumar, J. (2019). Interleukin-6 contributes to the development of anemia in juvenile CKD. Kidney international reports, 4(3), 470-483.

shu, T., Jing, C., Lv, Z., Xie, Y., Xu, J., & Wu, J. (2015). Hepcidin in tumor‐related iron deficiency anemia and tumor‐related anemia of chronic disease: pathogenic mechanisms and diagnosis. European journal of haematology, 94(1), 67-73.

Raj, D. S. (2009). Role of interleukin-6 in the anemia of chronic disease. Paper presented at the Seminars in arthritis and rheumatism

Fujiwara, T., Okamoto, K., Niikuni, R., Takahashi, K., Okitsu, Y., Fukuhara, N., . . . Nakamura, Y. (2014). Effect of 5-aminolevulinic acid on erythropoiesis: a preclinical in vitro characterization for the treatment of congenital sideroblastic anemia. Biochemical and biophysical research communications, 454(1), 102-108.

Tilg, H., Ulmer, H., Kaser, A., & Weiss, G. (2002). Role of IL-10 for induction of anemia during inflammation. The Journal of Immunology, 169(4), 2204-2209

Nussenblatt, V., Mukasa, G., Grace, A.M., Garrett, N.E. and Semba, R.D. (2001). Anemia and Interleukin-10, Tumor Necrosis Factor Alpha, and Erythropoietin Levels among Children with Acute, Uncomplicated Plasmodium falciparum Malaria. Clinical and Diagnostic Laboratory Immunolology, 8(6): 1164–1170

Ludwiczek, S., Aigner, E., Theurl, I. and Weiss, G. (2003). Cytokine-mediated regulation of iron transport in human monocytic cells. Blood, 101(10):4148-4154

Bergman, M., Bessler, H., Salman, H., Siomin, D., Straussberg, R., & Djaldetti, M. (2014). In vitro cytokine production in patients with iron deficiency anemia. Clinical Immunology, 113(3), 340-344.

Zaghloul, A., Al-Bukhari, T., Bajuaifer, N., Shalaby, M., Al-Pakistani, H., Halawani, S. H., . . . Wassif, G. A. (2016). Introduction of new formulas and evaluation of the previous red blood cell indices and formulas in the differentiation between beta thalassemia trait and iron deficiency anemia in the Makkah region. Hematology, 21(6), 351-358.

Kuvibidila, S., & Warrier, R. P. (2004). Differential effects of iron deficiency and underfeeding on serum levels of interleukin-10, interleukin-12p40, and interferon-gamma in mice. Cytokine, 26(2), 73-81.

Published
2021-07-30
How to Cite
Rabaa Khaled Abdel Salam, & Saria Naji Mohsin. (2021). Estimation of IL-6 and IL-10 Levels in Patients with Iron Deficiency Anemia. International Journal for Research in Applied Sciences and Biotechnology, 8(4), 124-128. https://doi.org/10.31033/ijrasb.8.4.19
Section
Articles