Vesicular Arbuscular Mycorrhiza Association Augments Antioxidant Activity of Vitex negundo Leaves
The study was conducted to evaluate the effect of Vesicular Arbuscular Mycorrhiza treatment on antioxidant activity of leaves of Vitex negundo. Mycorrhiza spores were added to the plants at different time intervals the plants were catergoried as T1, T2, T3 and T4 according to treatments. T1 plants are control while T2, T3 and T4 plants received treatment of spores for one time, twice at intervals of 30 days and every day for 15 days till harvesting. After harvesting and processing of the leaves, methanolic extracts were prepared by maceration and designated as MEVNT1, MEVNT2, MEVNT3 and MEVNT4. The extracts were studied for quantitative analysis of antioxidant phytoconstituents (total phenolic, flavonoid and ascorbic acid contents) and in vitro antioxidant property in 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay, hydrogen peroxide scavenging assay, reducing power assay, anti-lipid peroxidation assay. Methanol extract of V. negundo leaves and ascorbic acid showed significant scavenging of (p < 0.05) DPPH free radicals, reducing potential, hydroxyl radical scavenging and anti-lipid peroxidation effect. Among these parameters, IC50 value of MEVNT4 was lowest among all extracts in almost all parameters studied besides its high content of antioxidant phytochemicals. The results indicated that Mycorrhiza treatment caused marked elevation on antioxidant status of the plant. It further states that single treatment or treatment of long intermittent duration may have lesser potential to affect the antioxidant profile that the continuous treatment period. This suggested that the treatment have tendency to augment the concentration or effect of antioxidant present in the leaves.
Aswar, P.B., Khadabadi, S.S., Kuchekar, B.S., Rajurkar, R.M., Saboo, S.S. and Javarkar, R.D. (2009). In Vitro Evaluation of Anti-Bacterial and Anti-Fungal Activity of Vitex nigundo (Verbenaceae). Ethnobotanical Leaflets, 13, 962-967.
Bolan NS. (1991). A critical review on the role of mycorrhizal fungi in the uptake of phosphorus by plants. Journal of Plant and Soil, 134, 189-207
Brand-Williams W, Cuvelier ME, Berset C. Use of a Free Radical Method to Evaluate antioxidant Activity. Lebensm-Wiss u.-Technol 1995; 28: 25-30.
Chopra RN, Nayar SL, Chopra IC. (1986) Glossary of Indian Medicinal Plants (Including the Supplement). Council of Scientific and Industrial Research, New Delhi. Classification, pharmacological, biochemical effects and therapeutic potential. Indian J Pharmacol 33, 2-16.
Khare CP. (2004) Encyclopedia of India Medicinal plants. Spinger Verlange, Berline, Heidelberge, NEW YORK, 474-476.
Marinova D, Ribarova F, Atanasova M. (2005) Total phenolics and flavonoids in Bulgarian fruits and vegetables. J Univ Chem Tech Metall, 40(3), 255-260.
Meena, A. K., Niranjan, U.S., Rao, M.M., Padhi, M.M. and Ramesh Babu. (2011). A review of the important chemical constituents and medicinal uses of Vitex genus. Asian Journal of Traditional Medicines, 6(2).
Muthuswamy, U., Kuppusamy, A., Nandagopi, U., Thirumalaisamy, S. and Varadharajan S. (2012). Protective effect of the leaves of Vitex negundo against ethanol-induced cerebral oxidative stress in rats. Tanzania J Health Res., 14(1). 1-1.
Ohkawa H, Ohishi N, Yagi K. (2001). Assay for lipid peroxides in animal tissue by thiobarbituric acid reaction. Anal Biochem , 95, 351–358.
Omaye ST, Turnbull JD and Sauberlich HE. (1979). Selected methods for the determination of ascorbic acid in animal cells, tissues and fluids. Methods in Enzymol, 62.
Ono, M., Nishida, Y., Masuoka, C., Li, J., Okawa, M., Ikeda, T. and Nohara, T. (2004). Lignan derivatives and a norditerpene from the seeds of Vitex negundo. Journal of Natural Products, 67, 2073-2075.
Oyaizu M; Studies on product of browning reaction prepared from glucose amine. Jpn J Nutr 1986; 307–315.
Preethi, R., Devanathan, V.V. and Loganathan M. Antimicrobial and antioxidant efficacy of some medicinal plants against food borne pathogens. Advan. Biol Res. 2010; 4 (2): 122-125.
Rabeta, M. S. and An Nabil, Z. Total phenolic compounds and scavenging activity in Clitoria ternatea and Vitex negundo Linn. International Food Research Journal. 2013; 20(1): 495-500.
Rao Govinda , Suresh YS, Suresh CK, Suresh NS,RR Mallikarjunaiah , DJ Bagyraj. Vesicular arbuscular mycorhizae fungi in medicinal plants. Indian Journal of Phythopathology; 1989; 42, 476-478
Ruch RJ, Cheng SJ, Klaunig JE. Prevention of cytotoxicity and inhibition of intracellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogen 1989; 10:1003-1008.
Vishal, T. and Gupta, R.K.; Effect of Vitex negundo on oxidative stress. Indian journal of pharmacology; 2005; 37.
Chikkaswamy BK. And Paramanik RC, Effects of VAM and Biofertilizers on some Medicinal Plants, Int.J.Curr.Microbiol.App.Sci 2014; 3(6): 1016-1027
Jing, T.Y. Zhao X.Y. The improved pyrogallol method by using termination agent for
Superoxide dismutase measurement. Prog. Biochem. Biophys. 1995; 22, 84–86.
Thapa T, De UK, Chakraborty B. Association and root colonization of some medicinal plants with Arbuscular Mycorrhizal Fungi. Journal of Medicinal Plants Studies 2015; 3(2): 25-3.
Evelin H, Kapoor R. Arbuscular mycorrhizal symbiosis modulates antioxidant response in salt-stressed Trigonella foenum-graecum plants. Mycorrhiza. 2014; 24(3):197-208.
Khandelwal KR. Practical Pharmacognosy. 15th ed. Nirali Prakashan, Pune, 2006:149-156.
Singleton V, Orthofer R, Lamuela-Ravento's R. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. In: Packer L, editor. Oxidants and antioxidants, part A. Methods in Enzymology. Vol. 299. New York: Academic Press; 1999. pp. 152–178.
Pandey A, Kaushik A, Wanjari M, Dey YN, Jaiswal BS, Dhodi A., Antioxidant and anti-inflammatory activities of Aerva pseudotomentosa leaves; 2017; 55(1) 1688-1697.
Coyle JT, Puttfarcken P. (1993) Oxidative stress, glutamate, and neurodegenerative disorders. Science 262: 689–695
Copyright (c) 2018 International Journal for Research in Applied Sciences and Biotechnology
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.