Human Adenovirus Serotypes Efficiently Transducing HEK293 Cells: An In Vitro Propagation of HAdv

  • Mr. Utkalendu Suvendusekhar Samantaray Research Associate, Dept. of Vaccine Development, Virchow Biotech Pvt Ltd, Hyderabad, INDIA
  • Ms. Piyanki Santra BTech, MTech, Dept. of Biotechnology, KIIT University, Bhubaneswar, Odisha, INDIA
Keywords: Cells, Culture media, COVID-19, Adenovirus vector

Abstract

Generally, a gene which is inserted directly into a cell does not operate independently. Instead, the transmission of the gene is genetically modified by a biological messenger called a vector, consists of a transgene and a large DNA sequence as a backbone. Since they can deliver the new gene by infecting the cell, such viruses are also used as vectors. The adenovirus is a non-enveloped virus that can be tailored to transfer DNA to target cells, and it has sparked a lot of interest in the field, particularly in clinical trial therapy techniques. For the new age production of COVID-19 vaccine, development of different mammalian cell lines like HEK293 (most reliable growth and prosperity for transfection) and recombinant adenoviral vectors have become the first priority for biopharmaceutical giants and globally approved vaccine manufacturers to scale up their vaccine production. Adenoviruses have an icosahedral shape, with a protein coat encasing the viral double-stranded DNA genome. Because the adenovirus genome is relatively small, it's a good candidate for insertion of foreign DNA. The adenovirus E1A gene is deleted, and the virus loses its capacity to replicate. This ability can be restored during cell culture propagation by employing cells that produce the E1A protein, for example. Hence, in this mini research, I have shared an overview of the propagation of adenoviral vectors, i.e. recombinant adenovirus SARS CoV-2 vector in HEK-293 cell suspension culture.

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Author Biography

Mr. Utkalendu Suvendusekhar Samantaray, Research Associate, Dept. of Vaccine Development, Virchow Biotech Pvt Ltd, Hyderabad, INDIA

Mr. Utkalendu is working on monoclonal antibodies and vaccine development in biopharmaceutical industries as a major researcher and lead. He is a major key member in Gam-cov-vac for COVID-19 disease. Optimization of culture media, development of different mammalian cell lines like CHO, HEK293, virus culture, transfection, upstream development, downstream purification, etc. Mr. Utkalendu Suvendusekhar Samantaray has been completed his master's in biotechnology from MITS School of biotechnology affiliated with Utkal University. He has worked on many research papers including biochemistry, anti-oxidant development, plant growth microbes, nanotechnology, etc.

References

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Hoeben, R. C., & Uil, T. G. (2013). Adenovirus DNA Replication. Cold Spring Harbor Perspectives in Biology, 5(3), a013003. https://doi.org/10.1101/cshperspect.a013003

Lee, C. S., Bishop, E. S., Zhang, R., Yu, X., Farina, E. M., Yan, S., Zhao, C., Zeng, Z., Shu, Y., Wu, X., Lei, J., Li, Y., Zhang, W., Yang, C., Wu, K., Wu, Y., Ho, S., Athiviraham, A., Lee, M. J., . . . He, T. C. (2017). Adenovirus-mediated gene delivery: Potential applications for gene and cell-based therapies in the new era of personalized medicine. Genes & Diseases, 4(2), 43–63. https://doi.org/10.1016/j.gendis.2017.04.001

Published
2021-09-08
How to Cite
Samantaray, M. U. S., & Santra, M. P. (2021). Human Adenovirus Serotypes Efficiently Transducing HEK293 Cells: An In Vitro Propagation of HAdv. International Journal for Research in Applied Sciences and Biotechnology, 8(5), 17-21. https://doi.org/10.31033/ijrasb.8.5.3