Antioxidant Biosensor based on Deinococcus radiodurans Biofilm immobilized on Screen-printed Carbon Electrode (SPCE) Surface

  • Amalyah Febryanti
  • Sri Mulijani
  • Dyah Iswantini
  • Novik Nurhidayat
Keywords: antioxidant biosensor, biofilm, D. radiodurans, SOD, SPCE


Antioxidant biosensor based on D. radiodurans biofilm has been investigated in this research. The biofilm producing SOD enzymes were immobilized on SPCE surface. Optimization of experimental measurements were carried out by the response surface method. The optimum value obtained was at the buffer pH 7, suspension pH 6, and optical density (OD) 0.5. The morphology of SPCE surfaces was characterized by SEM. The optimum result was used to determinate analytical performance, including linearity, sensitivity, limit of detection (LOD), limit of quantity (LOQ), precision, selectivity, stability, and repeatability. Linearity was achieved in the xanthine concentration range of 0.1-0.6 mM with the equation y = 40.79x + 57.173 and R² = 0.99. The apparent Michaelis-Menten constant KMwas evaluated. It was found that the biosensor had alow KMof 40 μM. LOD and LOQ respectively 40.8 μM and 123.7 μM with sensitivity 40.79 μA mM-1. Precision showed that RSD was less than 5%. Stability was measured for 35 days andretained 90% of current for the period. Repeatability showedRSD ≤ 5%. The selectivity of this method still needs to increase. In conclusion, antioxidant biosensor based on D. radioduransbiofilm may be used to measure the capacity of antioxidant products practically.


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Prior R.L, Wu X, Schaich K. (2005). Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. J Agric Food Chem. 53(10): 4290-4302.

Roginsky V, Lissi E.A. (2005). Review of methods to determine chain-breaking antioxidant activity in food. Food Chemistry. 92(2): 235-254.

Photinon K, Chalermchart Y, Khanongnuch C, Wang SH, Liu C.C. (2010). A thick film sensor as a novel device for determination of polyphenols and their antioxidant capacity in white wine. Sensors. 10(3): 1670-1678.

Pavinatto F.J, Paschoal C.W, Arias A.C. (2014). Printed and flexible biosensor for antioxidants using interdigitated ink-jetted electrodes and gravure-deposited active layer. Biosensors And Bioelectronics. 67: 553-559.

Cabaj J, Jadwiga S, Nawakowska-Oleksy A. (2016). Tyrosinase biosensor for antioxidants based on semiconducting polymer support. Electroanalysis. 28: 1383-1390.

Monošíka R, Streanskýb M, Šturdík. (2012). Biosensors-classification, characterization and new trends. Electrochemistry. 5: 109-120.

Dhull V, Gahlaut A, Dilbaghi N, Hooda V. (2013). Acetylcholinesterase biosensors for electrochemical detection of organophosphorus compounds: a review, Biochem. Research Inter. 2013:1-18.

Akyilmaz E. & Dinçkaya E. (2005). An amperometric microbial biosensor development based on Candida tropicalis yeast cells for sensitive determination of ethanol. Biosensor and Bioelectronics. 20(7): 1263-1269.

Iswantini D, Trivadila, Nurhidayat N, Nurcholis W. (2013). Antioxidant Biosensor Using Microbe. Int Journal of Medical, Health, Biomedical, Bioengineering and Pharmaceutical Engineering. 7: 263-270.

Wijayanti. (2014). Antioxidant Biosensor using Superoxide Dismutase Enzyme of Deinococcus radiodurans Bacteria immobilized on Zeolite Nanoparticle. [Thesis]. Bogor Agricultural University, Bogor. [Indonesia].

Afifi MR. (2016). Superoxide Biosensor using Protein Extract of Deinococcus radiodurans immobilized with cross-linked by glutaraldehyde. [Thesis]. Bogor Agricultural University, Bogor. [Indonesia].

He Y. (2009). High cell density production of Deinococcus radiodurans under optimized conditions. J Ind Microbiol Biotechnol. 36(4): 539-546.

Holland A.D, Rothfuss H.M, Lidstrom M.E. (2006). Development of a defined medium supporting rapid growth for Deinococcus radiodurans and analysis of metabolic capacities. Appl Microbiol Biotechnol. 72(5): 1074–1082.

Iswantini D, Rachmatia R, Diana N.R, Nurhidayat N, Akhiruddin, Saprudin D. (2016). Activity and stability of biofilm uricase of Lactobacillus plantarum for uric acid biosensor. Earth and Envir. Science. 31: 1-16.

International Conference on Harmonisation. (2005). Validation of Analytical Procedures: Text and Methodology Q2(R1). New York (US): International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use. Available at Accessed 24 Januari 2018.

Slade D. & Radman M. (2011). Oxidative stress resistan CE in Deinococcus radiodurans. Microbiol. and Mol. Biol. Rev.75(1): 133-191.

Shukla S.K, Sankar G.G, Paraneeiswaran A, Subba A, Rao ST. (2014) Differential radio-tolerance of nutrition-induced morphotypes of Deinococcus radiodurans R1. Curr Microbio. 68(2): 225-247.

Daly M.J. (2009). A new perspective on radiation resistance based on Deinococcus radiodurans. Nat. Rev. Microbiol. 7(3): 237-245.

Brooks W. & Murray G. (1981). Nomenclature for Micrococcus radiodurans and other radiation-resistant cocci: Deinococcaceae fam. nov. and Deinococcus gen. nov., Including Five Species. Int J Syst Bacteriol. 31: 353-360.

Kolari M, Schmidt U, Kuismanen E, Salkinoja-Salonen MS. (2002). Firm but slippery attachment of Deinococcus geothermalis. J Bacteriol. 184:2473-2480.

Shukla S.K. & Rao S.T. (2015). Heavy metals-bioremediation by highly radioresistant Deinococcus radiodurans biofilm prospective use in nuclear reactor decontamination. Symposium on water chemistry and corrosion in nuclear power plants in Asia, Kalpakkam, India.

Hu Y, Zhang J, Ulstrup J. (2011). Investigation of Streptococcus mutans biofilm growth on modified Au (111)-surfaces using AFM and electrochemistry. J Electroanal Chem. 656:41-49.

]Nanduri V, Sorokulova I.B, Samoylov A.M. (2007). Biosens. Bioelectron. 22: 986-992.

Pine Research. (2016). Screen-Printed Electrode Information Carbon and Ceramic Electrode Information. Available at Accessed 2 March 2017.

Baniukevic J, Kirlyte J, Ramanavicius A, Ramanaviciene A. (2013). Application of oriented and random antibody immobilization methods in immunosensor design. Sens. Actuators B. 189:217-223.

Sharma M.K, Goel A.K, Singh L, Rao V.K. (2006). Immunological Biosensor for Detection of Vibrio cholerae O1 in Environmental Water Samples. World J. Microbiol. Biotechnol. 22:1155-1159.

How to Cite
Amalyah Febryanti, Sri Mulijani, Dyah Iswantini, & Novik Nurhidayat. (2018). Antioxidant Biosensor based on Deinococcus radiodurans Biofilm immobilized on Screen-printed Carbon Electrode (SPCE) Surface. International Journal for Research in Applied Sciences and Biotechnology, 5(4), 1-7. Retrieved from