Isolation and Biochemical Characterization of Indole-3-Acetic Acid (IAA) Produced in Pseudomonas Sp. Isolated from Rhizospheric Soil

  • Mr. Utkalendu Suvendusekhar Samantaray Research Associate, Department of Vaccine Development, Virchow Biotech Pvt. Ltd, Hyderabad, INDIA.
  • Ms. Swapnarani Sethi Department of Biotechnology, MITS School of Biotechnology, Bhubaneswar, Odisha, INDIA
Keywords: Indole-3-acetic acid, Biochemical characterization, Pseudomonas Sp., Rhizospheric soil

Abstract

A phytohormone is a molecule that controls the development of plants. Auxin and cytokines, which may be acquired as synthetic compounds or produced by microorganisms, which promote plant growth, are employed to enhance crop yield. Indole-3-Acetic Acid (IAA) is a key phytohormone that controls a number of physiological processes in plants. Indole-3-acetic acid (IAA), the most prevalent endogenous auxin, is involved in stem elongation and root formation. Auxin levels are usually higher in the rhizosphere. The possibility of rhizosphere bacteria to encourage direct plant development has been recognised as a crucial component of auxin (IAA) production. They promote the growth of lateral roots, which increases the surface area available for nutrition absorption and enhances nutrient and water absorption from the soil. The objective of this research is to isolate, characterise, and identify bacteria that produce indole acetic acid in rhizospheric soil. Bacterial colonies were isolated using the serial dillution technique on nutrient agar medium from rhizospheric soil samples of a rice crop. Five rhizospheric bacterial isolates (RIPB-1 to RIPB-30) were identified as effective Indole acetic acid makers after qualitative screening. The quantity of Indole acetic acid produced by five bacterial strains was tested for up to 120 hours. The amount of Indole acetic acid they could generate ranged from 1 to 11.2 g/ml. The bacterial strain RIPB-20 (11.2 g/ml) generated the highest Indole acetic acid after 72 hours of incubation, followed by RIPB-14. Based on morphological, physiological, and biochemical features, the bacterial isolate RIPB-20 was tentatively identified as Bacillus sp. Finally, the finding shows that the bacteria, which have capacity to produce Indole acetic acid, are good biofertilizer inoculants for growth of plant. 

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Author Biography

Mr. Utkalendu Suvendusekhar Samantaray, Research Associate, Department of Vaccine Development, Virchow Biotech Pvt. Ltd, Hyderabad, INDIA.

Mr. Utkalendu is working on monoclonal antibodies and vaccine development in biopharmaceutical industries as a major researcher and lead. He is a major key member in Gam-cov-vac for COVID-19 disease. Optimization of culture media, development of different mammalian cell lines like CHO, HEK293, virus culture, transfection, upstream development, downstream purification, etc. Mr. Utkalendu Suvendusekhar Samantaray has been completed his master's in biotechnology from MITS School of biotechnology affiliated with Utkal University. He has worked on many research papers including biochemistry, anti-oxidant development, plant growth microbes, nanotechnology, etc.

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Published
2021-07-05
How to Cite
Mr. Utkalendu Suvendusekhar Samantaray, & Ms. Swapnarani Sethi. (2021). Isolation and Biochemical Characterization of Indole-3-Acetic Acid (IAA) Produced in Pseudomonas Sp. Isolated from Rhizospheric Soil. International Journal for Research in Applied Sciences and Biotechnology, 8(4), 1-14. https://doi.org/10.31033/ijrasb.8.4.1
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Articles