Optimization of Modified Murashige and Skoog (MS) Medium Promoting Efficient Callus Induction in Zinnia elegans

  • Mr. Utkalendu Suvendusekhar Samantaray Research Associate, Dept. of Vaccine Development, Virchow Biotech Pvt Ltd, Hyderabad, INDIA
  • Ms. Tanima Singh Department of Biotechnology, MITS School of biotechnology, Odisha, INDIA
Keywords: Callus, Murashige and Skoog, 2,4 dichlorophenoxyacetic acid


Zinnia (Zinnia elegans Jacq.) occupies a pristine place in the garden for its bright colours and long flowering period. Zinnias are propagated by seeds hence genetic variation and a tendency for stock plants to decline in vigor over time is high. Young internodal explants of this species were grown on modified Murashige and Skoog (MS) medium supplemented with various growth hormones to evaluate callus induction. 2,4 dichlorophenoxyacetic acid (2,4-D) at 1.0 mg/L was shown to be the best choice for a high percentage of callus induction (70-73%) and a rapid growth rate of viable and healthy callus. A large-scale and quick callus generation protocol was developed in the lab and might be used for in-vitro micropropagation.


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Author Biography

Mr. Utkalendu Suvendusekhar Samantaray, Research Associate, Dept. of Vaccine Development, Virchow Biotech Pvt Ltd, Hyderabad, INDIA

Mr. Utkalendu is working on monoclonal antibodies and vaccine development in biopharmaceutical industries as a major researcher and lead. He is a major key member in Gam-cov-vac for COVID-19 disease. Optimization of culture media, development of different mammalian cell lines like CHO, HEK293, virus culture, transfection, upstream development, downstream purification, etc. Mr. Utkalendu Suvendusekhar Samantaray has been completed his master's in biotechnology from MITS School of biotechnology affiliated with Utkal University. He has worked on many research papers including biochemistry, anti-oxidant development, plant growth microbes, nanotechnology, etc.


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How to Cite
Mr. Utkalendu Suvendusekhar Samantaray, & Ms. Tanima Singh. (2021). Optimization of Modified Murashige and Skoog (MS) Medium Promoting Efficient Callus Induction in Zinnia elegans. International Journal for Research in Applied Sciences and Biotechnology, 8(3), 202-211. https://doi.org/10.31033/ijrasb.8.3.25