Optimization of Modified Murashige and Skoog (MS) Medium Promoting Efficient Callus Induction in Zinnia elegans
Zinnia (Zinnia elegans Jacq.) occupies a pristine place in the garden for its bright colours and long flowering period. Zinnias are propagated by seeds hence genetic variation and a tendency for stock plants to decline in vigor over time is high. Young internodal explants of this species were grown on modified Murashige and Skoog (MS) medium supplemented with various growth hormones to evaluate callus induction. 2,4 dichlorophenoxyacetic acid (2,4-D) at 1.0 mg/L was shown to be the best choice for a high percentage of callus induction (70-73%) and a rapid growth rate of viable and healthy callus. A large-scale and quick callus generation protocol was developed in the lab and might be used for in-vitro micropropagation.
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