Optimization of Modified Murashige and Skoog (MS) Medium Promoting Efficient Callus Induction in Zinnia elegans

  • Mr. Utkalendu Suvendusekhar Samantaray Research Associate, Dept. of Vaccine Development, Virchow Biotech Pvt Ltd, Hyderabad, INDIA
  • Ms. Tanima Singh Department of Biotechnology, MITS School of biotechnology, Odisha, INDIA
Keywords: Callus, Murashige and Skoog, 2,4 dichlorophenoxyacetic acid

Abstract

Zinnia (Zinnia elegans Jacq.) occupies a pristine place in the garden for its bright colours and long flowering period. Zinnias are propagated by seeds hence genetic variation and a tendency for stock plants to decline in vigor over time is high. Young internodal explants of this species were grown on modified Murashige and Skoog (MS) medium supplemented with various growth hormones to evaluate callus induction. 2,4 dichlorophenoxyacetic acid (2,4-D) at 1.0 mg/L was shown to be the best choice for a high percentage of callus induction (70-73%) and a rapid growth rate of viable and healthy callus. A large-scale and quick callus generation protocol was developed in the lab and might be used for in-vitro micropropagation.

Downloads

Download data is not yet available.

Author Biography

Mr. Utkalendu Suvendusekhar Samantaray, Research Associate, Dept. of Vaccine Development, Virchow Biotech Pvt Ltd, Hyderabad, INDIA

Mr. Utkalendu is working on monoclonal antibodies and vaccine development in biopharmaceutical industries as a major researcher and lead. He is a major key member in Gam-cov-vac for COVID-19 disease. Optimization of culture media, development of different mammalian cell lines like CHO, HEK293, virus culture, transfection, upstream development, downstream purification, etc. Mr. Utkalendu Suvendusekhar Samantaray has been completed his master's in biotechnology from MITS School of biotechnology affiliated with Utkal University. He has worked on many research papers including biochemistry, anti-oxidant development, plant growth microbes, nanotechnology, etc.

References

Ahuja, M.R. (1965). Genetic control of tumor formation in higher plants. Q. Rev. Biol. 40: 329–340.

Akiyoshi, D.E., Morris, R.O., Hinz, R., Mischke, B.S., Kosuge, T., Garfinkel, D.J., Gordon, M.P., and Nester, E.W. (1983). Cytokinin/ auxin balance in crown gall tumors is regulated by specific loci in the T-DNA. Proc. Natl. Acad. Sci. USA 80: 407–411.

Atta, R., Laurens, L., Boucheron-Dubuisson, E., Guivarc’h, A., Carnero, E., Giraudat-Pautot, V., Rech, P., and Chriqui, D. (2009). Pluripotency of Arabidopsis xylem pericycle underlies shoot regeneration from root and hypocotyl explants grown in vitro. Plant J. 57: 626–644.

Barash, I., and Manulis-Sasson, S. (2007). Virulence mechanisms and host specificity of gall-forming Pantoeaagglomerans. Trends Microbiol.15: 538–545.

Bostock, R.M., and Stermer, B.A. (1989). Perspectives on wound healing in resistance to pathogens.Annu. Rev. Phytopathol. 27: 343–371.

Chiappetta, A., Fambrini, M., Petrarulo, M., Rapparini, F., Michelotti, V., Bruno, L., Greco, M., Baraldi, R., Salvini, M., Pugliesi, C., and Bitonti, M.B. (2009). Ectopic expression of LEAFY COTYLEDON1-LIKE gene and localized auxin accumulation mark

Frank, M., Rupp, H.-M., Prinsen, E., Motyka, V., Van Onckelen, H., and Schmülling, T. (2000). Hormone autotrophic growth and differentiation identifies mutant lines of Arabidopsis with altered cytokinin and auxin content or signaling. Plant Physiol. 122: 721–729.

Gautheret, R. (1939). Sur la possibilité de réaliser la culture indéfinie des tissues de tubercules de carotte. C. R. Soc. Biol. Paris 208: 118–120

Glick, B.R. (1995). The enhancement of plant growth by free-living bacteria.Can. J. Microbiol.41: 109–117.

Hu, Y., Bao, F., and Li, J. (2000). Promotive effect of brassinosteroids on cell division involves a distinct CycD3-induction pathway in Arabidopsis. Plant J. 24: 693–701.

Ichikawa, T., and Syōno, K. (1988). Tumorization-redifferentiation system of tobacco genetic tumor. Plant Cell Physiol. 29: 1373–1378.

Ichikawa, T., and Syōno, K. (1991). Tobacco genetic tumors. Plant Cell Physiol. 32: 1123–1128.

Iwase, A., Mitsuda, N., Koyama, T., Hiratsu, K., Kojima, M., Arai, T., Inoue, Y., Seki, M., Sakakibara, H., Sugimoto, K., and Ohme-Takagi, M. (2011a). The AP2/ERF transcription factor WIND1 controls cell dedifferentiation in Arabidopsis. Curr. Biol. 21: 508–514.

Jammes, F., Lecomte, P., de Almeida-Engler, J., Bitton, F., Martin- Magniette, M.L., Renou, J.P., Abad, P., and Favery, B. (2005). Genome-wide expression profiling of the host response to root-knotnematode infection in Arabidopsis. Plant J. 44: 447–458.

Kirthikar KR and Basu BD., Indian Medicinal Plants, Dehradun:International book distributor, 2006, 1364-1345.

Lee, C. (1955). Anatomical changes in sweet clover shootsinfectedwith Wound-Tumor Virus. Am. J. Bot. 42: 693–698

Malinowski, R., Smith, J.A., Fleming, A.J., Scholes, J.D., and Rolfe, S.A. (2012). Gall formation in club root-Infected Arabidopsis is caused by an increase in the host's current meristematic activity, but it is not infectious essential forthe pathogen's growth cycle has come to an end. plant J. 71: 226–238.

Manjamalai A, Satyajit SR, Guruvavayoorappan.C and Berlin Grace VM., Analysis of phytochemical constituents and anti-microbial activity, Global Journal of Biotech. andBiochem, 2010, 5(2), 120-128.

Manulis, S., Haviv-Chesner, A., Brandl, M.T., Lindow, S.E., and Barash, I. (1998). Differential involvement of indole-3-acetic acidbiosynthetic pathways in pathogenicity and epiphytic fitness of Erwiniaherbicolapv. gypsophilae. Mol. Plant Microbe Interact. 11: 634–642.

Mathew KM., Flora of Tamilnadu-carnatic. Trichirapalli, St. Josephs College, 1983, 392 685-687.

Miyajima. D. 1996. Germination of zinnia seed with and without pericarp. Seed Science & Tech., 24: 465-473.

Miyajima, D. 2000. Effects of watering frequency, grain size of vermiculite and various temperatures on the emergence of zinnia (Zinnia violacea) seeds with or without pericarps. J. Japanese Soc. hort. Sci., 69: 60-62.

Nagata, T., and Takebe, I. (1971).Plating of isolated tobacco mesophyll protoplasts on agar medium.Planta 99: 12–20.

Neely, D. (1979). Tree wounds and wound closure. J. Arboriculture 5:135–140.

r Research 2009, 15(17), 5435-5444

Sacristan, M., and Melchers, G. (1977). Regeneration of plants from “habituated” and “Agrobacterium-transformed” single-cell clones of tobacco. Mol. Gen. Genet. 152: 111–117.

Sitbon, F., Sundberg, B., Olsson, O., and Sandberg, G. (1991). Free and conjugated indoleacetic acid (IAA) contents in transgenic tobacco plants expressing the iaaM and iaaH IAA biosynthesis genes from Agrobacterium tumefaciens. Plant Physiol. 95: 480–485.

Sharan A. K, M. Kumar, R Singh, Neha, A. Kishor, G. D. Sharma and C. Jee, 2011. Effect of vermicompost on manifestation of pesticide action on growth of Zinnia elegans. African J. Biotech. 10(36): 6991-6996.

Skoog, F., and Miller, C.O. (1957). Chemical regulation of growth and organ formation in plant tissues cultured in vitro. Symp. Soc. Exp. Biol. 11: 118–130.

Steward, F.C., Mapes, M.O., and Mears, K. (1958). Growth and organized development of cultured cells.II. Organization in cultures grown from freely suspended cells. Am. J. Bot. 45: 705–708.

Stobbe, H., Schmitt, U., Eckstein, D., and Dujesiefken, D. (2002). Developmental stages and fine structure of surface callus formed after debarking of living lime trees (Tilia sp.). Ann. Bot. (Lond.) 89: 773–782.

Sugimoto, K., Jiao, Y., and Meyerowitz, E.M. (2010). Arabidopsis regeneration from multiple tissues occurs via a root development pathway. Dev. Cell 18: 463–471.

Tooker, J.F., Rohr, J.R., Abrahamson, W.G., and De Moraes, C.M. (2008). Gall insects can avoid and alter indirect plant defenses. NewPhytol.178: 657–671.

Tsai CH, Lin FM,. Yang YC. Lee MT and Cha TL., Androgen receptor supressing activity and

Udagawa, M., Aoki, S., and Syono, K. (2004). Expression analysis of the NgORF13 promoter during the development of tobacco genetic tumors. Plant Cell Physiol. 45: 1023–1031.

Published
2021-06-22
How to Cite
Mr. Utkalendu Suvendusekhar Samantaray, & Ms. Tanima Singh. (2021). Optimization of Modified Murashige and Skoog (MS) Medium Promoting Efficient Callus Induction in Zinnia elegans. International Journal for Research in Applied Sciences and Biotechnology, 8(3), 202-211. https://doi.org/10.31033/ijrasb.8.3.25
Section
Articles