Quantitative Analysis of Nutrients and Biochemical Evaluation in Pomfret Fish (Pampus argenteus) During Frozen Storage

  • Mr. Utkalendu suvendusekhar Samantaray Research Associate, Dept. of Vaccine Development, Virchow Biotech Pvt Ltd, Hyderabad, INDIA
  • Ms. Swagatika Tripathy Department of Biotechnology, Maharaja Sriram Chandra Bhanja Deo University, Baripada, Odisha
Keywords: Biochemical Evaluation, coronary heart disease, Nutrients

Abstract

Marine fish are well-known for being a high-quality protein source having high concentration of essential amino acids.  It has high concentration of mono unsaturated and poly unsaturated fatty acids, which may aid in the optimization of lipid profiles and the reduction of the risk of coronary heart disease (CHD). The goal of this study was to estimate the nutritional and biochemical status of raw sea fish Pampus argenteus after 30 days of frozen storage at -200C with 15-day intervals. Nutrient study showed a decrease in protein and lipid content. The changes of hydrogen peroxide and oxidized lipid products were estimated in the muscle tissue during fresh and storage condition. Results indicate that during storage the oxidative stress increased. An antioxidant enzyme (superoxide dismutase, smutase, catalase, glutathione peroxidase) measurement was determined. The increased amount of oxidative stress during fish storage is shown by the differential activity of antioxidant enzymes. The amount of protein in fish varies slightly between species and even within species. Fish is high in protein, vitamins, minerals, and omega-3 fatty acids, which are essential for brain development (Spencer et al., 1971; Jacylin et al., 2010). A well-balanced diet consists variety of fish that can help in children's growth and development as well as their heart health (Jinadasa, 2014).

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Author Biography

Mr. Utkalendu suvendusekhar Samantaray, Research Associate, Dept. of Vaccine Development, Virchow Biotech Pvt Ltd, Hyderabad, INDIA

Mr. Utkalendu is working on monoclonal antibodies and vaccine development in biopharmaceutical industries as a major researcher and lead. He is a major key member in Gam-cov-vac for COVID-19 disease. Optimization of culture media, development of different mammalian cell lines like CHO, HEK293, virus culture, transfection, upstream development, downstream purification, etc. Mr. Utkalendu Suvendusekhar Samantaray has been completed his master's in biotechnology from MITS School of biotechnology affiliated with Utkal University. He has worked on many research papers including biochemistry, anti-oxidant development, plant growth microbes, nanotechnology, etc.

References

Abbas KA, Mohamed M, Jamilah B, Ebrahimian., (2008) A review on correlations between fish freshness and pH during cold storage., Am. J. Biochem.Biotech.4: 416-421.

Aebi H., (1984) “Catalase in vitro” Methods Enzymol.105:121-127.

Allen R.G., (1991) Oxygen reactive species and antioxidant responses during development: The metabolic paradox of cellular differentiation.Proc.Soc.Exp.Biol.Med.196:117-129.

Amiard, JC, A-Triquet, C, Barka S, Pellerin J and Rainbow PS., (2006) Metallothioniens in aquatic invertebrates: Their role in metal detoxification and their role in metal detoxification and their use as biomarkers. Aquat. Toxicol.76:160-202.

Amer J, Ghoti H, Koren A, Levin C., (2006) RBC, platelets and polymorphonuclear neutrophils of patients with sickle-cell anaemia. Brit. J. Haem. 132(1):108-113.

Anthony JA, Roby DD, Turco KR., (2000) Lipid content and energy density of forage fishes from the northern Gulf of Alaska. J. Exp. Mar. Bio. Ecol. 248: 53-78.

Atli G, Alptekin I, Tukel S, Canli M., (2006) Response of catalase activity to Ag+,Cd2+,Cr2+,Cu2+ and Zn2+ in five tissues of freshwater fish Oreochromisniloticus. Comp. Biochem. Physiol.143:218-224.

Association of Official Analytical Chemists (AOAC) (2000) Official methods of analysis.In: Horowitz W, ed. Seventeenth edition. Gaithersburg: Association of Official Anal. Chem.

Basha S P, Rani U A., (2003) Cadmium- induced antioxidant defense mechanism in freshwater teleocystOreochromismossambicus(Tilapia). Ecotoxicol and Environ. 56:218-221.

Beers B, Sizer W., (1952) Aspectrophometric method for measuring the breakdown of hydrogen peroxide by catalase. J. Biol. Chem. 195:133-139.

Berntssen M.H.G., Aatland, A. and Handy, R.D., (2003) Chronicdietry mercury exposure causes oxidative stress, brain lesions, and altered behavior in Atlantic salmon (Salmosalar) parr. Aquat.Toxicol. 65:55-72.

Blaha L., Kopp R, Imkova K, Mare J., (2004) Oxidative Stress Biomarkers are modulated in Silver carp (Hypophthalmichthysmolitrix Val.) exposed to Microcystin-producing cyanobacterial water bloom. Actavet.Brno.73:477-482.

Bradford M.M., (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding.Anal.Biochem. 72:248-254.

Bonomini F, Rezzani R., (2008) Atherosclerosis and oxidative stress. Histol.Histopathol. 23(3): 381-390.

Burchi F., Fanzo, J., and Frison E., (2011) The role of food and nutrition system approaches in tackling hidden hunger. Int J. Environ. Res. Pub. Heal. 8(2):358-373.

Cohen G, Dembiac D, Marcus J., (1934) Measurement of catalase activity in tissue extracts. Anal.Biochem. 134(1):30-38.

Das K, Samanta L., Chainy G. B. N., (2000) A modified spectrophotometric assay of superoxide dismutase using nitrite formation of superoxide radicals. Ind. J. Biochem.Biophys. 37:201-204.

Davies K JA., (1995) Oxidative stress, the paradox of aerobic life. In: Rice-Evans C, Halliwell B, Land GG, editors. Free radical and Oxidative Stress: Environ. D. Fd. Add. London: Portland Press. Biochem. Soc Symp. 61:1–31.

Di Giulio RT, Meyer JN., (2008) Reactive oxygen species and oxidative stress. In: Di Giulio RT, Hinton DE, editors.Toxicol. Fish. Boca Raton: CRC Press, Taylor and Francis GroupDorval J, Leblond V, Deblois C. 17:273–324.

Eufemia NA, Collier TK, Stein JE, Watson DE, Di Giulio RT., (1997) Biochemical responses to sediment associated contaminants in brown bull head (Ameriurusnebulosus) from the NiagaraRiverecosystem.Ecotoxicol. 6:13–34.

Filho D.W., (1996).Fish antioxidant defense a comparative approach. Brazilian J. Med. Bio.Res., 29:1735-1742.

Filho D.W., (2007).Reactive Oxygen species, antioxidants and fish mitochondria: Front Biosci. 12:1229-1237.

Gornell A G. Bardwill C S. David M M., (1949) Method for measuring proteins with biuret .J. Biol. Chem. 177:751-766.

Gunzler W A, Kremmers H, Flohe L., (1974) An improved coupled test procedure for glutathione peroxidase in blood. J. Clini. Chem. Biochem. 12:444-448.

Habig W, Pabst M, Jakoby W., (1974) Glutathione S-transferases: the first enzymatic step in mercapturic formation. J. Biol. Chem. 22:7130-7139.

Halliwell B., (2007) Oxidative stress and cancer: have we moved forward? Biochem J. 401(1):1-11.

Halliwell B., Gutteridge, J.M.C., (2007) Free radicals in biology and medicine, 4th ed. Oxford University Press, Oxford, United Kingdom.

Hedge J.E. and Hofreiter B.T., (1962) In: Carbohydrate Chemistry, 17 (Eds. Whistler R.L. and Be Miller, J.N.), Academic Press, New York.

Hermann A.C., Millard, P., Blake. (2004) Development of a respiratory burst assay using zebra fish kidneys and embryos. J. Immunol. Methods 292:119-129.

Hwang O., (2013) Role of oxidative stress in Parkinson’s disease.Exp. Neurobiol. 22(1):11-7.

Ingram J., (2011) A food systems approach to researching food security and its interactions with global environmental change.Food Sec. 3(4):417-431.

Jos A., Pichardo, S., Prieto Ana, I., Repetto, G., Vazquez Carmen, M., Moreno, I., Camean, (2005).Toxic cyano-bacterial cells containing microcystins induce oxidative stress in exposed tilapia fish under laboratory conditions. AquatToxicol. 72:261-271.

Jaclyn M, Stacey JB, Ashley SR., (2010) Omega-3 fatty acids and pregnancy. Rev ObstetGynecol. 3(4):163-171.

JinadasaBKKK ., (2014) Determination of Quality of Marine Fishes Based on Total Volatile Base Nitrogen test (TVB-N). Nat. Sci. 12(5):106-111.

Joseph N, Zhang-JamesY, Perl A, Faraone SV., (2015) Oxidative Stress and ADHD: A Meta analysis” Atten Disord. 19(11):915-924.

K. J. Whittle., (1995) Sea food from producer to consumer, integrated approach to quality in Sea Food from Producer to Consumer, Integrated Approach to Quality. Proceedings of The International Seafood Conference on The 25Th Anniversary of WEFTA, J. B. Luten, T. Borrosen, and J. Oehlenschlager, Eds., Elseveer, Netherlands, Amsterdam.

Kadomura K. Nakashima T., Kurachi M., Yamaguchi (2016) Production of reactive oxygen species (ROS) by devil stinger during embryogenesis. Fish Shell fish Immunol. 21:209-214.

Kamal M, Islam MN, Mansur MA, Bhuiyan MAL., (1996 ) Biochemical and sensory evaluation of hilsa fish (Tenualosailisha) during frozen storage. In. J M. Sci. 25:320-323.

Kono Y, Fridovich I., (1982) Superoxide radical inhibits catalase. J. Biol. Chem. 257: 5751– 5754.

Kossmann C.H., (1988) How do fish get their vital vitamin C? Fish Farm Int. 5:30.

Kurachi T., M., Yamaguchi. (2009) Production of reactive oxygen species (ROS) by devil stinger during embryogenesis. Fish Shell fish Immunol. 21:209-214.

Lavigne C., F. Tremblay, G. Asselin, H. Jacques, and Marette A., (2001) Prevention of skeletal muscle insulin resistance dietary cod protein in high fat-fed rats. Am. J. Physio.-Endocrino. Metabo. 281:E62–E71.

Lennon SV., Martin SJ., Cotter TG., (1991) Dose-dependent induction of apoptosis in human tumour cell lines by widely diverging stimuli. Cell Prolif. 24:203-214.

Marklund S, Marklund G., (1974) Involvment of the superoxide anion radical in the auto oxidation of pyrogallol and a convient assay for superoxide dismutase. Eup. J. Biochem. 47:469-474.

Machala M, Dusek L, Hilscherova K, Kubinova R, Jurajda P, Neca J et al., (2001) Determination and multivariate statistical analysis of biochemical responses to environmental contaminants in feral freshwater fish Leuciscuscephalus L. Environ Toxicol. Chem. 250:1141-1148.

MCCarthy J F, Shugart L R., (1992) Biological marker of environmental contamination, Boca Raton FL:Lewis.

MehwishFaheem, Khalid Parvez L.,(2018) Oxidative stress and histopathologic biomarkers of exposure to bis-phenol A in the freshwater fish Ctenopharyngdonidella. Braz. J. Pharm. Sci.vol.53.

Messaoudi I, Barahoumi S, Said K, and Kerken A., (2009) Study on sensitivity to cadmium of marine fish Salarariabasilisca (piscesBlennidae).J.Environ. Sci, 21:20-1624.

Mezes M.,(1986) Investigation of vitamin E content and lipid peroxidation in blood and tissues of common carp. Aquatic Hung 5:71-78.

Meyer M., Schreck, R.Muller, J. Baeuerie, P. A., (1994) Oxidative stress, Cell Activation and viral infection. Mol.Cell Biol. Upd. 217-235.

Mizuguchi., (1998) Single photon counting. Methods. Mol. Biol., 108: 37Y-55Y.

Mohanty BP, Sankar TV, Ganguly S, Mahanty A, Anandan R, ChakrabartyKet al., (2016b) .DHA, EPA content and fatty acids profile of 39 food fishes of India. Biomed Res. 18(1):137-148.

Mukundan M.K., Radhakrishnan A.G., James, M A., & Nair, M.R., (1981) Fish.Technol.18: 129-132

Nugent R., (2011) Bringing agriculture to the table: How agriculture and food can play a role in preventing chronic disease. Chicago, USA: The Chicago Council on Global Affairs.

Ohkawa H, Ohishi N, Yagi K., (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 95:351-358.

Ouellet V. J. Marois, S. J. Weisnagel, and Jacques H., (2007) Dietary cod protein improves insulin sensitivity in insulin-resistant men and women. Diab. Care. 30:2816–2821.

Payne SA, Johnson BA, Otto RS., (1999) Proximate composition of some northeastern Pacific forage fish species. Fish Oceano gr 8: 159-177.

Pick E., and Keisari Y., (1981) Superoxide anion and hydrogen peroxide production by chemically elicited peritoneal macrophages-induction by multiple nonphagocytic stimuli. Cell Immunol. 59:301-318.

Ramond A, Ribuot C., (2013) Oxidative stress mediates cardiac infarction aggravation induced by intermittent hypoxia. Fundam.Clin.Pharmacol. 27(3):252-61

Rodriguez-Ariza A, AlhamaJ, Diaz-MendezFM, Lopez-Barea J., (1999) Contentof8-oxodG in chromosomal DNA of Sparusaurata fish as a biomarker of oxidative stress and environmental pollution. Mutat Res. 438:97–107.

Roche H, Boge G., (1993) Effects of Cu, Zn and Cr salts on antioxidant enzyme activities in vitro of red blood cells of a marine fish Dicentrarchuslabrax.Toxicol.in Vitro. 7:623-629.

Schafer FQ, Buettner GR., (2001) Redox environment of the cell as viewed through the redox state of the glutathione disulfide /glutathione couple. Free Radiac. Biol. Med. 30(11): 1191-212.

Simopoulos A.P., (2003) Importance of the ratio of omega6/omega3 essential fattyacids: Evolutionary aspects. Biomed.Pharmacother. 56(8):365-379.

Singh N, Dhalla AK, Senevirtane C, Singal PK., (1995).Oxidative Stress and heart failure. Mol. Cell.Biochem. 147(1-2):77-81.

Slaininova L.P. MotaA .A. Hermes-Lima M., (2001) Oxidative stress and antioxidant defenses in gold fish during anoxia and reoxygeneration. Am J. Physiol. Regul. Integr. Comp. Physiol. 280(1):100-107.

Sobal J., Kettel Khan L., and Bisogni C., (1998) A conceptual model of the food and nutrition system.Soc. Sci. Med. 47(7):853-863.

Spencer H, Samachson Fowler J, Kulka MJ., (1971) Availability in man of protein and minerals from fish protein concentrate. Am J.Clin.Nutr 24(3):311-317.

Stansby ME., (1962) In Industrial fishery technology,edited by ME Stansby, (Reinhold Publishing Corporation, London), Pp 340.

Stolze K, Nohl H., (1994) Effect of xenobiotics on the respiratory activity of rat heart mitochondria and the concomitant formation of superoxide radicals. Environ. Toxicol. Chem. 13:499–502.

Sun P., Yin, F. and Tang, B., (2017) Effects of acute handling stress on expression of Growth related genes in Pampusargenteus. J. W. Aqual.Soc. 48:166-179.

Taheri S A. A. Motallebi, A. Fazlara, Y. Aftabsavar, and S. P. Aubourg., (2012) Influence of vacuum packaging and long term storage on some quality parameters of cobia(Rachycentroncanadum) fillets during frozen storage. Ame-Euras J. Agri. Environ. Sci., 12(4):541–547.

Tenyanga N. H. M. Womenib, B. Tiencheub, Villeneuved P. and M. Lindere., (2017) Effect of refrigeration time on the lipid oxidation and fatty acid profiles of catfish (Arius maculatus) commercialized in Cameroon, Grasas Y Aceite 68(1).

Tsangaris C., Vergolyas M., Fountoulaki E., Nizheradze K., (2011a) Oxidative stress and genotoxicity biomarkers responses in Grey Mullet (Mugilcephalus) from a polluted environment in Saronikos Gulf, Greece. Arch. Environ. Contam.Toxicol. 61:482–490.

Tsangaris C., Vergolyas, M., Fountoulaki, E., Goncharuk, V.V., (2011b) Geno toxicity and oxidative stress biomarkers in Carassiusgibelioas endpoints for toxicity testing of Ukrainian polluted river waters. Ecotoxicol. Environ. Saf.74:2240– 2244

Turrens JF (2003) Mitochondrial Formation of reactive oxygen species.The Journal of Physiology. 552(2):335-44.

Valko M, Cronon, MT Mazur M, Telser J., (2007) Free radicals and antioxidant in normal physiological functions and human disease. Int.J.Biochem.Cell Biol. 39(1):44-84.

Valavanidis A, Vlahogianni T, Dassenakis M, Scoullos M., (2006) Molecular biomarkers of oxidative stress in aquatic organisms in relation to toxic environmental pollutants. Ecotoxicol Environ Saf. 64(2):178–189.

Venugopal V., (2002) Biosensors in fish production and quality control BiosensBioelectr. 17(3): 147-157.

Wills E.D.,(1969) Lipid peroxide formation in microsomes. General considerations.Biochem.J.113:315-324.

Winston GW, Di Gulio RT, (1991) Proxidant and antioxidant mechanism of and toxicityat the cellular level. Aquat Toxicol. 19:137-161.

Yang W. T. Li, J.andYue G. H., (2006) Multiplex genotyping of novel microsatellites from silver pomfret (Pampusargenteus) and cross-amplification in other pomfret species. Mol.Ecol. Notes. 6:1073-1075.

Yoshikawa T., (2002) A Guide to Free Radicals Part 2.Sentan IgakuSha,Tokyo,. J.M.A.J. 45(7):271-276.

Yin Fei, Peng, Shiming ,Sun, Peng, Shi Zhaohong., (2011) Effects of low salinity on antioxidant enzymes on in kidney and muscle in of juvenile silver pomfret. Acta. Ecologica. Sinica. 31(1):55-60.

Zaitsev VP., (1952) In preservation of fish products by refrigeration (Pischepromizdat, Moscow), Pp 232, (in Russian).

Zhao F., Dong, Y., Zhuang, P., Zhang, T., Zhang, L., & Shi, Z., (2011) Genetic diversity of silver pomfret (Pampus argenteus) in the Southern yellow and East ChinaSeas. Biochem. Syst. Eco. 39(2):145-150.

Published
2021-06-17
How to Cite
Samantaray, M. U. suvendusekhar, & Tripathy, M. S. (2021). Quantitative Analysis of Nutrients and Biochemical Evaluation in Pomfret Fish (Pampus argenteus) During Frozen Storage. International Journal for Research in Applied Sciences and Biotechnology, 8(3), 180-195. https://doi.org/10.31033/ijrasb.8.3.23
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Articles