Biochemical Analysis of Phenolics Compounds Accumulated During Developing Stages of Wheatgrass
The current study was carried out to evaluate the accumulation of phenolic compounds in distinct developmental stages of Wheatgrass (Tritium aestivum). The wheatgrass was grown in the container/tray in the standard condition in the laboratory space provided by the department. Overnight soaked seeds were then sowed in the soil containers for 25 days. The accumulation of phenolics compounds was qualitatively and quantitatively checked in the leaves at 5, 10,15,20,25 days. Phenolics compounds were sequentially extracted in methanol, n-hexane, and acetone. For qualitative analysis, various biochemical tests were carried out and phenolics compounds were quantitatively estimated by Folin-Ciocalteu reagent. Among three solvents methanol extract was found to accumulate phenolics compounds in increasing concentration at various developmental stages i.e. 5th days - 0.7135 mg/ml, 10th days 1.2614 mg/ml, 15th days- 1.1244 mg/ml; 20th days - 1.8093 mg/ml and 25th days - 4.0693 mg/ml. On the qualitative check, methanol extract was found to be positive for tannins, terpenoids, and glycosides. These phenolics could be analyzed for antidiabetic and antimicrobial activities are the future perspective of this study.
 Velderrain-Rodriguez, G.R; Palafox-Carlos, H; Wall-Medrano, A; Ayala Zavala, J.F; Chen, C-Y.O.; Robles-Sanchez, M.; Astiazaran-Garcia, H.; Alvarez-Parrilla, (2014) E.; Gonzalez-Aguilar, G.A. Phenolic compounds: Their journey after intake. Food Funct. 2014, 5, 189-197
 Ali Khoddami, Meredith A. Wilkes, and Thomas H. Roberts (2013) Techniques for analysis of plant phenolic compound. et.al. 2011 >.molecules>v.18(2).2013 Feb PMC6270361
 Klepacka, J., Elzbieta Gujska and Joanna Michalak; (2011) Phenolic compounds as cultivator – and variety- distinguishing factors in some plant product. Springer, plant food hum nutr (2011) 66:64-69
 Babbar, N.; Oberoi, H.S.; Sandhu, S.K.; Bhargav, V.K.(2014) Influence of different solvents in extraction of phenolic compounds from vegetable residues and their evaluation as natural sources of antioxidants. J. Food Sci. Technol. 2014, 51, 2568–2575.
 Acamovic, T.; Brooker, J.D. (2005) Biochemistry of plant secondary metabolites and their effects in animals. Published online by Cambrige University Press: 07 March 2007.in Proc Nutr Soc. 2005 Volume 64.
 Edreva, A.; Velikova, V.; Tsonev, T.; Dagnon, S.; Gürel, A.L.; Aktas, L. (2007) Stress- protective role of secondary metabolites: Diversity of functionsand mechanisms. Gen. Appl. Plant. Physiol. 2008, 34, 67–70
 Randhir, R.; Lin Y.T.; Shetty, K. (2004) Stimulation of phynolics, antioxidant and antimicrobial activities in dark germinated mung bean sprouts in response to peptide and phytochemical elicitors. Elsevier science direct
 Vattem, D.A.; Randhir, R.; Shetty, K.; (2005) Cranberry Phenolics-mediated antioxidant enzyme response in oxydatively stressed porcine muscles. Elsevier, Process biochemistry vol.40 issue 06 may (2006)
 Heima, K.E.; Tagliaferroa, A.R.; Bobila, D.J (2002) Flavonoid antioxidants: Chemistry metabolism and structure-activity relationships. Journal of Nutritional Biochemistry.
 Sasikala. S, Radhaisri. S (2015) Analysis of total phenol in developmental Nutraceutical by uv-vis spectrophotometry. International journal of science and research (2319-7064).
 Harborne JB, Baxter H, Moss GP.(1999) Phytochemical Dictionary: A hand book of Bioactive compounds from plants. Food and Nutrition sciences, vol.3 No.6
 King A. Young G. (1999.) Characteristics and occurrence of phenolic phytochemicals. J Am Diet Association 1999 feb.
 Tanaka T, Shnimizu M, Moriwaki H. (2012) Cancer chemoprevention by carotenoids. Molecules. 2012;17:3202–3242
 Nardini M, Natella F, Scaccini C. (2007) Role of dietary polyphenols in platelet aggregation. A review of the supplementation studies. Platelets 2007; 18: 224-43.
 Dubick MA, Omaye ST. (2002) Evidence for grape, wine and tea polyphenols as modulators of atherosclerosis and ischemic heart disease in humans. J Nutraceut Functional & Med Foods 2001; 3:67-93.
 Cragg GM, Newman DJ. Natural product drug discovery in the next millennium. Pharm Biol 2001;39 Suppl 1:8-17
Copyright (c) 2021 International Journal for Research in Applied Sciences and Biotechnology
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.