Inhibition of HepG-2 Cells (Liver Cancer Cell Line) Viability by 3-Hydroxypyridine-2-Carboxaldehyde N(4)-Methylthiosemicarbazone

  • Edrees Khan Rahmatzada Ghalib University, Kabul, AFGHANISTAN
  • Prof. Paras Nath Yadav Tribhuvan University, Kathmandu, NEPAL
  • Dr. Yuba Raj Pokharel South Asian University, Akbar Bhawan, Chanakyapuri, New Delhi-110021, New Delhi, INDIA
Keywords: Thiosemicarbazone, viability, HepG-2, antiviral, antibacterial, antifungal, and anticancer, wound healing assay, colony formation assay, CV assay, JC-1 staining, western blot, 3-Hydroxypyridine-2-Carboxaldehyde N(4)-Methylthiosemicarbazone

Abstract

Thiosemicarbazone have the antiviral, antibacterial, antifungal, and anticancer effects. 3-OH-Me-TSC inhibited the cell viability of HepG-2 cells by CV assay in a concentration dependent manner (control, 1μM, 3μM, 10μM, 30μM, and 100μM) with IC50 value of 9.587622μM. Further colony formation assay demonstrated that 3-OH-Me-TSC inhibits colony number and size of HepG-2. Wound healing assay exhibited that 3-OH-Me-TSC inhibit the migration of HepG-2 cells. DAPI staining showed that 3-OH-Me-TSC inhibited proliferation of HepG-2 cells in 30μM and 100μM concentrations respectively. 3-OH-Me-TSC inhibited VEGF, p38 alpha, C-JUN, BECN-1, ERK, NF-KB, in HepG-2 cells. We found that 3-OH-Me-TSC inhibit proliferation of HepG-2 cells by inhibiting MAPK signaling pathway, 3-OH-Me-TSC can be developed as future chemotherapeutic agent for treatment of hepatocellular carcinoma after the evaluation of this compounds in more cancer cells an in vivo model.

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References

Weinberg, R. A. (2014). The Biology of Cancer. New York, NY: Garland Science.

Hanahan, Douglas, and Robert A. Weinberg. “Hallmarks of Cancer: The Next Generation.” Cell 144, no. 5 (March 2011): 646–74.

El–Serag, Hashem B., and K. Lenhard Rudolph. "Hepatocellular Carcinoma: Epidemiology and Molecular Carcinogenesis." Gastroenterology 132, no. 7 (2007): 2557-576. Doi:10.1053/j.gastro.2007.04.061.

Khan, Tahmeena, Rumana Ahmad, Seema Joshi, and A R Khan. “Anticancer Potential of Metal Thiosemicarbazone Complexes: A Review,” 2015, 11.

“Preparation and Characterization of Ni(II) and Mn(II) Complexes of Semicarbazone and Thiosemicarbazone of m- Hydroxy Benzaldehyde and p- Hydroxy Benzaldehyde.” Accessed May 8, 2019.

Khan, T., Ahmad, R., Azad, I., Raza, S., Joshi, S. And Khan, A. (2018). Computer-aided drug design and virtual screening of targeted combinatorial libraries of mixed-ligand transition metal complexes of 2-butanone thiosemicarbazone. Computational Biology and Chemistry, 75, pp.178- 195.

“The Hallmarks of Cancer.” In Wikipedia, November 21, 2018. Https://en.wikipedia.org/w/index.php?Title=The_Hallmarks_of_Cancer&oldid=870015458.

Di Bisceglie, Adrian M. “Hepatitis B And Hepatocellular Carcinoma.” Hepatology (Baltimore, Md.) 49, no. 5 Suppl (May 2009): S56–60.

Banerjee A, Ray RB, Ray R. Oncogenic Potential of Hepatitis C Virus Proteins. Viruses. 2010;2(9):2108-2133. Doi:10.3390/v2092108

Singh, Mandeep Kumar, Bhrigu Kumar Das, Sandeep Choudhary, Deepak Gupta, and Umesh K. Patil. “Diabetes and Hepatocellular Carcinoma: A Pathophysiological Link and Pharmacological Management.” Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie 106 (October 2018): 991–1002.

Kew MC. Hepatic Iron Overload and Hepatocellular Carcinoma. Liver Cancer. 2014; 3(1):31-40. Doi:10.1159/000343856

Bialecki, Eldad S., and Adrian M. Di Bisceglie. “Diagnosis of Hepatocellular Carcinoma.” HPB : The Official Journal of the International Hepato Pancreato Biliary Association 7, no. 1 (2005): 26–34.

Ryder, S. D. “Guidelines for the Diagnosis and Treatment of Hepatocellular Carcinoma (HCC) in Adults.” Gut 52, no. Suppl 3 (May 1, 2003): iii1–8.

”Guidelines for the Diagnosis and Treatment of Hepatocellular Carcinoma (HCC) in Adults.” Gut 52, no. Suppl 3 (May 1, 2003): iii1–8. Https://doi.org/10.1136/gut.52.suppl_3.iii1.

Tejeda-Maldonado, Javier, Ignacio García-Juárez, Jonathan Aguirre-Valadez, Adrián González-Aguirre, Mario Vilatobá-Chapa, Alejandra Armengol-Alonso, Francisco Escobar- Penagos, Aldo Torre, Juan Francisco Sánchez-Ávila, and Diego Luis Carrillo-Pérez. “Diagnosis and Treatment of Hepatocellular Carcinoma: An Update.” World Journal of Hepatology 7, no. 3 (March 27, 2015): 362–76. Https://doi.org/10.4254/wjh.v7.i3.362.

Zhu, Ran Xu, Wai-Kay Seto, Ching-Lung Lai, and Man-Fung Yuen. “Epidemiology of Hepatocellular Carcinoma in the Asia-Pacific Region.” Gut and Liver 10, no. 3 (May 2016): 332– 39. Https://doi.org/10.5009/gnl15257.

“BECN1 Beclin 1 [Homo Sapiens (Human)] - Gene - NCBI.” Accessed May 8, 2019. Https://www.ncbi.nlm.nih.gov/gene/8678

“C-Jun - Wikipedia.” Accessed May 8, 2019. Https://en.wikipedia.org/wiki/C-

“Miller DM, Thomas SD, Islam A, Muench D, Sedoris K. C-Myc and Cancer Metabolism. Clin Cancer Res. 2012;18(20):5546-5553. Doi:10.1158/1078-0432.CCR-12-

“Koul HK, Pal M, Koul S. Role of p38 MAP Kinase Signal Transduction in Solid Tumors. Genes Cancer. 2013;4(9-10):342-359. Doi:10.1177/194760191350795148

Mccubrey JA, Steelman LS, Chappell WH, et al. Roles of the raf/mek/erk pathway in cell growth, malignant transformation and drug resistance. Biochim Biophys Acta. 2007;1773(8):1263-1284. Doi:10.1016/j.bbamcr.2006.10.001

Mccubrey JA, Steelman LS, Chappell WH, et al. Roles of the raf/mek/erk pathway in cell growth, malignant transformation and drug resistance. Biochim Biophys Acta. 2007;1773(8):1263-1284. Doi:10.1016/j.bbamcr.2006.10.001

“Angela M. Duffy, David J. Bouchier-Hayes, and Judith H. Harmey. Vascular Endothelial Growth Factor (VEGF) and Its Role in Non-Endothelial Cells: Autocrine Signalling by VEGF

EMMA YASINSKI, et al. Exosomes from Blood Carry RNA Signatures of Liver Cancer.2019

CAROLYN WILKE, et al. Exosomes Linked to Cancer Spread from Chemoresistant Tumors in Mice. Jan 10, 2019.

Published
2021-03-24
How to Cite
Edrees Khan Rahmatzada, Prof. Paras Nath Yadav, & Dr. Yuba Raj Pokharel. (2021). Inhibition of HepG-2 Cells (Liver Cancer Cell Line) Viability by 3-Hydroxypyridine-2-Carboxaldehyde N(4)-Methylthiosemicarbazone. International Journal for Research in Applied Sciences and Biotechnology, 8(2), 88-97. https://doi.org/10.31033/ijrasb.8.2.11
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Articles