Laboratory Protocols for Isolation and Identification of Toxigenic Strains of Vibrio cholerae: A Review

  • Bijay Kumar Shrestha Department of Microbiology, Central Campus of Technology, Tribhuvan University, Hattishar, Dharan, Nepal
  • Manita Tumbahangphe Department of Microbiology, Central Campus of Technology, Tribhuvan University, Hattishar, Dharan, Nepal
  • Jenish Shakya Department of Microbiology, Central Campus of Technology, Tribhuvan University, Hattishar, Dharan, Nepal
  • Sujata Chauhan Department of Microbiology, Central Campus of Technology, Tribhuvan University, Hattishar, Dharan, Nepal
  • Bidhya Dhungana Department of Microbiology, Central Campus of Technology, Tribhuvan University, Hattishar, Dharan, Nepal
  • Romika Shrestha Department of Microbiology, Central Campus of Technology, Tribhuvan University, Hattishar, Dharan, Nepal
  • Jyoti Limbu Department of Microbiology, Central Campus of Technology, Tribhuvan University, Hattishar, Dharan, Nepal
  • Kabiraj Khadka Department of Microbiology, Central Campus of Technology, Tribhuvan University, Hattishar, Dharan, Nepal
  • Anu Rai Department of Microbiology, Central Campus of Technology, Tribhuvan University, Hattishar, Dharan, Nepal
Keywords: Vibrio cholerae, Culture, Pathogenesis, Epidemiology, Faecal sample, food, sewage, water, PCR

Abstract

Vibrio cholerae is a pathogenic bacteria that accounts for diarrheal illness which can be life-threatening in lack of treatment and accounts for great morbidity and mortality. The common mode of transmission includes fecal-oral route transmission mediated through contaminated food and water. The strains 01 and 0139 have been associated with epidemic outbreak of cholera with severe clinical manifestation whereas the infection caused by non O1 and non O139 strains are recognized as non-epidemic cholera characterized by mild-severe diarrheal syndrome without epidemic potential. Laboratory diagnosis through Culture and determining Antimicrobial susceptibility tests are recommended for diagnosis of cholera in all clinical laboratory settings. The simple conventional laboratory diagnosis through culture should be effective for diagnosis of cholera in the resource (PCR) limited laboratories. However, Vibrio cholerae in VBNC (Viable but non-culturable) state can only be detected through molecular methods. Therefore, molecular methods need to be extended in all epidemiological laboratories for preventing misidentification of Vibrio cholerae especially from environmental samples. Epidemic outbreak of cholera has explained the need of immediate preparedness with preventive, diagnosis and curative improvement in all health sectors. Therefore, immediate health and sanitation related awareness are necessary for prompt control of the disease before it leads community to global burden.

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Published
2020-12-04
How to Cite
Kumar Shrestha, B., Tumbahangphe, M., Shakya, J., Chauhan, S., Dhungana, B., Shrestha, R., Limbu, J., Khadka, K., & Rai, A. (2020). Laboratory Protocols for Isolation and Identification of Toxigenic Strains of Vibrio cholerae: A Review. International Journal for Research in Applied Sciences and Biotechnology, 7(6), 181-189. https://doi.org/10.31033/ijrasb.7.6.26