Review Article: Bioseparation

  • Mohmmed Lefta Atala
Keywords: Bioseparation, Gel chromatography, Ion exchange chromatography, Affinity chromatography

Abstract

The Bio separation is stringent to the area of engineering for biochemistry. All bio separation operation should be suits to detach, purifying, or regain the coveted product. The bio separation depends on, essential property involved thermo constancy, solubility, diffusion, shipment, isoelectric pH, and other things. The purifying of biological products is get by a bio separations operations include a lengthy series of procedures, and all procedure needs the employ single or more unity operations, like filtrate, extraction, chromatography and desiccation. The Filtering isolate a components depending to volume while a liquid solute flux during a pored midst down a compressing differential. Super filtration of a protein solute at fixed size is completed with the addendum of H2O or buffer to the feed in an process named diafiltration. Fluid chromatography is a form of absorption operation, which is, depends on the affinity of different solution for specified form of solid. The generality efficiency chromatography process employ each the adsorption sites ready. While chromatography to purifying a protein is scaled up from the laboratory level, knowing of the laboratory elution profile is useful in interpreting the great level elution profile.

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References

[1] Wang, X., Hunter, A. K., & Mozier, N. M. (2009). Host cell proteins in biologics development: Identification, quantitation and risk assessment. Biotechnology and Bioengineering, 103(3), 446–458. https://doi.org/10.1002/bit.22304
[2] Spriestersbach, A., Kubicek, J., Schäfer, F., Block, H., & Maertens, B. (2015). Purification of His-Tagged Proteins. In Methods in Enzymology (Vol. 559, pp. 1–15). Elsevier. https://doi.org/10.1016/bs.mie.2014.11.003
[3] Scopes, R. K. (1994). Protein Purification. Springer New York. https://doi.org/10.1007/978-1-4757-2333-5
[4] Spriestersbach, A., Kubicek, J., Schäfer, F., Block, H., & Maertens, B. (2015). Purification of His-Tagged Proteins. In Methods in Enzymology (Vol. 559, pp. 1–15). Elsevier. https://doi.org/10.1016/bs.mie.2014.11.003
[5] Garrett RH, Grisham CM. (2013). Biochemistry (5th ed.). Belmont, CA: Brooks/Cole, Cengage Learning. p. 108. ISBN 9781133106296. OCLC 1066452448.
[6] Morgan, T. J., & Kandiyoti, R. (2014). Pyrolysis of Coals and Biomass: Analysis of Thermal Breakdown and Its Products. Chemical Reviews, 114(3), 1547–1607. https://doi.org/10.1021/cr400194p
[7] Skoog, D.A. Principles of Instrumental Analysis, 6th ed.; Thompson Brooks/Cole: Belmont, California, 2006, Chapter 28.
[8] Knill, C. J., & Kennedy, J. F. (2000). Polymer Synthesis and Characterization: A Laboratory Manual; S.R. Sandler, W. Karo, J.-A. Bonesteel, E.M. Pearce (Eds.); San Diego: Academic Press, 1998, xvii+212 pages, ISBN 0-12-618240-X, $39.95. Carbohydrate Polymers, 41(2), 220–221. https://doi.org/10.1016/S0144-8617(99)00135-6
[9] Ninfa, Alexander J., David Ballou P., and Marilee Benore. (2010). Fundamental Laboratory Approaches for Biochemistry and Biotechnology. Hoboken, NJ: John Wiley.
[10] Ninfa, Alexander; Ballou, David; Benore, Marilee. (2009). Fundamental Laboratory Approaches for Biochemistry and Biotechnology. Wiley. pp. 143–145.
[11] Principles of Ion Exchange Chromatography. separations.us.tosohbioscience.com. Retrieved 1 May 2018.
[12] Handbook for Monitoring Industrial wastewater. Environmental Protection Agency (USA). August 1973. Retrieved 30 July 2016.
[13] Da̧browski, A., Hubicki, Z., Podkościelny, P., & Robens, E. (2004). Selective removal of the heavy metal ions from waters and industrial wastewaters by ion-exchange method. Chemosphere, 56(2), 91–106. https://doi.org/10.1016/j.chemosphere.2004.03.006
[14] Luqman, ;Mohammad, and Inamuddin. (2012). Ion Exchange Technology II. Springer Netherlands. p. 1. ISBN 978-94-007-4026-6.
[15] Fritz, James S. (1987). Ion chromatography. Analytical Chemistry. 59 (4): 335A–344A.
[16] Siegel, Miles. (1997). Rapid purification of small molecule libraries by ion exchange chromatography. Tetrahedron Letters. 38(19): 3357–3358.
[17] Neubauer, Kenneth. (2016). Advantages and Disadvantages of Different Column Types for Speciation Analysis by LC-ICP-MS. spectroscopyonline.com. Retrieved 9 May 2016.
[18] Aizpurua-Olaizola, O., Sastre Torano, J., Pukin, A., Fu, O., Boons, G. J., de Jong, G. J., & Pieters, R. J. (2018). Affinity capillary electrophoresis for the assessment of binding affinity of carbohydrate-based cholera toxin inhibitors. ELECTROPHORESIS, 39(2), 344–347. https://doi.org/10.1002/elps.201700207
[19] Ninfa, Alexander J.; Ballou, David P.; Benore, Marilee (2009). Fundamental Laboratory Approaches for Biochemistry and Biotechnology (2nd ed.). Wiley. p. 133.
[20] Introduction to Affinity Chromatography. bio-rad.com. Bio-Rad. 2020-09-14. Retrieved 2020-09-14.
[21] Zachariou, Michael. (2008). Affinity Chromatography: Methods and Protocols (2nd ed.). Totowa, NJ: Humana Press. ISBN 9781588296597
[22] Bonner, Philip L.R. (2007). Protein Purification (2nd ed.). Totowa, NJ: Taylor & Francis Group. ISBN 9780415385114
[23] Bonner, Philip L.R. (2007). Protein Purification (2nd ed.). Totowa, NJ: Taylor & Francis Group. ISBN 9780415385114.
[24] Aizpurua-Olaizola, O., Sastre Torano, J., Pukin, A., Fu, O., Boons, G. J., de Jong, G. J., & Pieters, R. J. (2018). Affinity capillary electrophoresis for the assessment of binding affinity of carbohydrate-based cholera toxin inhibitors. ELECTROPHORESIS, 39(2), 344–347. https://doi.org/10.1002/elps.201700207
[25] Baur, D., Angarita, M., Müller-Späth, T., Steinebach, F., & Morbidelli, M. (2016). Comparison of batch and continuous multi-column protein A capture processes by optimal design. Biotechnology Journal, 11(7), 920–931. https://doi.org/10.1002/biot.201500481
[26] University of Babylon/ College of Science /Dept. of Biology, Jaralla, E. M., N. Al-Dabbagh, N., M.Hameed, N., & Abdul -Hussain, N. (2014). Screening for Enzymatic Production Ability and Antimicrobial Activity of Actinomycetes Isolated from Soil in Hillah/Iraq. IOSR Journal of Pharmacy and Biological Sciences, 9(5), 42–47. https://doi.org/10.9790/3008-09564247
Published
2020-11-08
How to Cite
Mohmmed Lefta Atala. (2020). Review Article: Bioseparation. International Journal for Research in Applied Sciences and Biotechnology, 7(6), 63-67. https://doi.org/10.31033/ijrasb.7.6.10