Transferring of Hybrid F1 of Spring Wheat into Dihaploid Basis Using Andgrogen Thechnologies

  • Aqarahim Wasim
Keywords: Anthogenesis, Anther Culture, Embryogenesis, Regeneration

Abstract

Anther culture is the most technologically advanced method of obtaining doubled haploids. Dihaploid lines are required to speed up the breeding process. From 7 hybrids of first-generation (F1) of spring wheat, 15 dihaploid culture lines were obtained by anther culture. An average of 23.7 embryo-like structures was obtained on 150 anthers. The percentage of plant regeneration was 49.1%, of which there were 39.9% albino plants and 9.1% green plants. The percentage of spontaneous chromosome doubling among the obtained green plants was 35.7.

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References

Zheng, M. Y. (2003). Microspore culture in wheat (Triticum aestivum) – doubled haploid production via induced embryogenesis. Plant Cell, Tissue and Organ Culture, 73(3), 213–230. https://doi.org/10.1023/A:1023076213639

Pausheva, Z.P. (1988) Plant Cytology Practical Training. Agropromizdat, Moscow. Issue. 4. - S. 58-100.

Lantos, C., Weyen, J., Orsini, J. M., Gnad, H., Schlieter, B., Lein, V., Kontowski, S., Jacobi, A., MihÁly, R., Broughton, S., & Pauk, J. (2013). Efficient application of in vitro anther culture for different European winter wheat ( Triticum aestivum L.) breeding programmes. Plant Breeding, 132(2), 149–154. https://doi.org/10.1111/pbr.12032

Rubtsova, M., Gnad, H., Melzer, M., Weyen, J., & Gils, M. (2013). The auxins centrophenoxine and 2,4-D differ in their effects on non-directly induced chromosome doubling in anther culture of wheat (T. aestivum L.). Plant Biotechnology Reports, 7(3), 247–255. https://doi.org/10.1007/s11816-012-0256-x

Ismagul A., Bashabaeva B.M., Iskakova G., Abugalieva A.I., Elibay S., Kenenbaev S.B. (2013) Culture of isolated wheat microspores. Toolkit, - Almaty, 19.

Published
2020-11-01
How to Cite
Aqarahim Wasim. (2020). Transferring of Hybrid F1 of Spring Wheat into Dihaploid Basis Using Andgrogen Thechnologies. International Journal for Research in Applied Sciences and Biotechnology, 7(6), 9-11. https://doi.org/10.31033/ijrasb.7.6.2