Transferring of Hybrid F1 of Spring Wheat into Dihaploid Basis Using Andgrogen Thechnologies

Aqarahim Wasim

doi:10.31033/ijrasb.7.6.2

Aqarahim Wasim

DOI: https://doi.org/10.31033/ijrasb.7.6.2

Keywords: Anthogenesis, Anther Culture, Embryogenesis, Regeneration

Abstract

Anther culture is the most technologically advanced method of obtaining doubled haploids. Dihaploid lines are required to speed up the breeding process. From 7 hybrids of first-generation (F1) of spring wheat, 15 dihaploid culture lines were obtained by anther culture. An average of 23.7 embryo-like structures was obtained on 150 anthers. The percentage of plant regeneration was 49.1%, of which there were 39.9% albino plants and 9.1% green plants. The percentage of spontaneous chromosome doubling among the obtained green plants was 35.7.

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