Transferring of Hybrid F1 of Spring Wheat into Dihaploid Basis Using Andgrogen Thechnologies
Anther culture is the most technologically advanced method of obtaining doubled haploids. Dihaploid lines are required to speed up the breeding process. From 7 hybrids of first-generation (F1) of spring wheat, 15 dihaploid culture lines were obtained by anther culture. An average of 23.7 embryo-like structures was obtained on 150 anthers. The percentage of plant regeneration was 49.1%, of which there were 39.9% albino plants and 9.1% green plants. The percentage of spontaneous chromosome doubling among the obtained green plants was 35.7.
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