Industrially Important Enzyme and Plant Growth Promoter Potential of Soil Actinomycetes

  • Y. H. K. I. S. Gunasinghe University of Kelaniya
  • E. A. A. D. Edirisinghe
Keywords: Soil, Actinobacteria, Enzyme, IAA


Enzymes and plant growth promoters have been found out to be the most widely used bacterial secondary metabolites, in industrial processes. Approximately, 10,000 bioactive metabolites which is 45% of the all bioactive microbial metabolites discovered, are produced by Actinobacteria. Therefore, they are considered as one of the most valuable microbial groups in biotechnology. In this study, the enzymes and Indole Acetic Acid (IAA) production capability and efficiency of soil Actinomycetes were explored. The enzymes production capability of 16 soil Actinomycetes isolates was tested. The number of isolates produced amylase, protease, L-asparaginase, cellulase, and lipase were 16, 8, 16, 14, and onerespectively. IAA production capability of the 16 soil-Actinomycetes isolates wasdetermined both qualitatively and quantitatively. Ten isolates out of 16 produced IAA at the concentration range of 5-66.15 µg/mL. Conditions, such as L-tryptophan concentration, pH ofthe medium of IAA production, and time duration of the fermentation were optimized in order to enhance the IAA production performance of Actinomycetes.



Download data is not yet available.


Ameur, H. and Ghoul, M. (2012). Screening of Actinomycetes Producing Antibacterial Substances and Indole Acetic Acid (IAA) and Optimization of Growth and IAA Production Conditions in Streptomyces sp. SF5. International Journal of Pharmaceutical & Biological Archives, 3(3), pp. 545–551.

Bano, N., & Musarrat, J. (2003). Characterization of a New Pseudomonas aeruginosa Strain NJ-15 as a Potential Biocontrol Agent. Current Microbiology, 46(5), 324–328.

Barati, B., & Sadegh Amiri, I. (2015). In Silico Engineering of Disulphide Bonds to Produce Stable Cellulase. Springer Singapore.

Praveen Kumar, P., Preetam Raj, J. P., Nimal Christhudas, I. V. S., Sagaya Jansi, R., Murugan, N., Agastian, P., Arunachalam, C., & Ali Alharbi, S. (2015). Screening of Actinomycetes for Enzyme and Antimicrobial Activities from the Soil Sediments of Northern Tamil Nadu, South India. Journal of Biologically Active Products from Nature, 5(1), 58–70.

Ball, A. S., & Mccarthy, A. J. (1988). Sacchariiication of Straw by Actinomycete Enzymes. Microbiology, 134(8), 2139–2147.

Cardenas, F., Alvarez, E., De Castro-Alvarez, M. S., Sánchez-Montero, J. M., Elson, S., & Sinisterra, J. V. (2001). Three New Lipases from Actinomycetes and Their Use in Organic Reactions. Biocatalysis and Biotransformation, 19(4), 315–329.

Chavan, D. V, Mulaje, S. S. and Mohalkar, R. Y. (2013). A Review on actinomycetes and their biotechnological applications. International Journal of pharmaceutical sciences and research, 4(5), pp. 1730–1742.

Costacurta, A., & Vanderleyden, J. (1995). Synthesis of phytohormones by plant-associated bacteria. Critical Reviews in Microbiology, 21(1), 1–18.

Datta, C., & Basu, P. S. (2000). Indole acetic acid production by a Rhizobium species from root nodules of a leguminous shrub, Cajanus cajan. Microbiological Research, 155(2), 123–127.

Ghosh, S., & Basu, P. S. (2006). Production and metabolism of indole acetic acid in roots and root nodules of Phaseolus mungo. Microbiological Research, 161(4), 362–366.

Nathan, K. V., Rebecca, L. J., & Mugam, P. A. (2017). Optimization of protease enzyme production by marine actinomycetes. International Journal of Pharma and Bio Sciences, 8(3).

Khamna, S., Yokota, A., & Lumyong, S. (2009). L-asparaginase production by actinomycetes isolated from some Thai medicinal plant rhizosphere soils. International Journal of Integrative Biology, 6(1), 22-26.

Manulis, S., Shafrir, H., Epstein, E., Lichter, A., & Barash, I. (1994). Biosynthesis of indole-3-acetic acid via the indole-3-acetamide pathway in Streptomyces spp. Microbiology, 140(5), 1045–1050.

Manulis, S., Shafrir, H., Epstein, E., Lichter, A., & Barash, I. (1994). Biosynthesis of indole-3-acetic acid via the indole-3-acetamide pathway in Streptomyces spp. Microbiology, 140(5), 1045–1050.

University of Babylon/ College of Science /Dept. of Biology, Jaralla, E. M., N. Al-Dabbagh, N., M.Hameed, N., & Abdul -Hussain, N. (2014). Screening for Enzymatic Production Ability and Antimicrobial Activity of Actinomycetes Isolated from Soil in Hillah/Iraq. IOSR Journal of Pharmacy and Biological Sciences, 9(5), 42–47.

Nafis, A., Raklami, A., Bechtaoui, N., El Khalloufi, F., El Alaoui, A., Glick, B. R., Hafidi, M., Kouisni, L., Ouhdouch, Y., & Hassani, L. (2019). Actinobacteria from Extreme Niches in Morocco and Their Plant Growth-Promoting Potentials. Diversity, 11(8), 139.

Narayana, K. J., Prabhakar, P., Vijayalakshmi, M., Venkateswarlu, Y., & Krishna, P. S. (2007). Biological activity of phenylpropionic acid isolated from a terrestrial Streptomycetes. Polish journal of microbiology, 56(3), 191–197.

Mustafa, O., A., U. T., & Cem, A. (2004). Antibacterial activity of some actinomycetes isolated from farming soils of Turkey. African Journal of Biotechnology, 3(9), 441–446.

Prakash, D., Nawani, N., Prakash, M., Bodas, M., Mandal, A., Khetmalas, M., & Kapadnis, B. (2013). Actinomycetes: A Repertory of Green Catalysts with a Potential Revenue Resource. BioMed Research International, 2013, 1–8.

Ranjani, A., Dhanasekaran, D., & Gopinath, P. M. (2016). An Introduction to Actinobacteria. In D. Dhanasekaran & Y. Jiang (Eds.), Actinobacteria—Basics and Biotechnological Applications. InTech.

Ryu, R. J., & Patten, C. L. (2008). Aromatic Amino Acid-Dependent Expression of Indole-3-Pyruvate Decarboxylase Is Regulated by TyrR in Enterobacter cloacae UW5. Journal of Bacteriology, 190(21), 7200–7208.

Dr. Chaitanya Kumar Jha, & Dr. Meenu Saraf. (2015). Plant growth promoting Rhizobacteria (PGPR): A review.

Savitri, Asthana, N. and Azmi, W. (2003). Microbial L-asparaginase: A potent antitumour enzyme. Indian Journal of Biotechnology, 2(2), 184–194.

Saxena, R. K. (1981). L-asparaginase and glutaminase activities in the culture filtrates of Aspergillus nidulans. Current science.

Selvam, K., Vishnupriya, B. and Bose, V. S. C. (2011). Screening and Quantification of Marine Actinomycetes Producing Industrial Enzymes Amylas, Cellulase and Lipase from South Coast of India. International Journal of Pharmaceutical & Biological Archives, 2(5), 1481–1487.

Kumar M, S., & K, S. (2011). Isolation and Purification of High Efficiency L-asparaginase by Quantitative Preparative Continuous-elution SDS PAGE Electrophoresis. Journal of Microbial & Biochemical Technology, 03(05).

Sethi, S., Datta, A., Gupta, B. L., & Gupta, S. (2013). Optimization of Cellulase Production from Bacteria Isolated from Soil. ISRN Biotechnology, 2013, 1–7.

Sharma, M., Dangi, P. and Choudhary, M. (2014). Actinomycetes : Source, Identification, and Their Applications. Int.J.Curr.Microbiol.App.Sci, 3(2): 801-832

Shirokikh, I. G., Zenova, G. M., Merzaeva, O. V., Lapygina, E. V., Batalova, G. A., & Lysak, L. V. (2007). Actinomycetes in the prokaryotic complex of the rhizosphere of oats in a soddy-podzolic soil. Eurasian Soil Science, 40(2), 158–162.

Sierra, G. (1957). A simple method for the detection of lipolytic activity of micro-organisms and some observations on the influence of the contact between cells and fatty substrates. Antonie van Leeuwenhoek, 23(1), 15–22.

Thakur, S. (2012). Lipases, Its sources, Properties and Applications: A Review. International Journal of Scientific & Engineering Research, 3(7), 1–29.

Yurekli, F., Geckil, H., & Topcuoglu, F. (2003). The synthesis of indole-3-acetic acid by the industrially important white-rot fungus Lentinus sajor-caju under different culture conditions. Mycological Research, 107(3), 305–309.

How to Cite
Y. H. K. I. S. Gunasinghe, & E. A. A. D. Edirisinghe. (2020). Industrially Important Enzyme and Plant Growth Promoter Potential of Soil Actinomycetes. International Journal for Research in Applied Sciences and Biotechnology, 7(6), 54-62.