Isolation and Pharmacological Activities of Curcumin from Curcuma longa L.

  • S. Mahalakshmi
  • V. S. Sruthi
  • N. G. Ramesh Babu
  • C. Sivaraj
  • P. Arumugam
Keywords: Curcuma longa, Antibacterial activity, Antioxidant activity, Antihaemolytic activity, DPPH


Curcuma longa commonly known as turmeric belongs to the family Zingiberaceae. It is mainly used for treating chronic diseases especially Diabetes mellitus. The present study aims at evaluating the anti-oxidant, anti-haemolytic, anti-angiogenesis and anti-bacterial activities of Curcumin fraction. Different antioxidant assays were carried out for evaluating the antioxidant capacity of aqueous extract of Curcuma longa. The maximum1, 1- diphenyl 2-picrylhydrazyl (DPPH) radical scavenging activity of curcumin fraction was 30.62±0.87 % at 60 µg/mL concentration. The inhibitory concentration (IC50) values of DPPH˙ radical scavenging activity was 97.97 µg/mL concentration. The maximum superoxide radical scavenging activity of Curcuma longa extract was 57.01±0.17 % at 60 µg/mL concentration and the inhibitory concentration (IC50) was 46.91 µg/mL concentration. The maximum anti-haemolytic activity of curcumin fraction was 44.53±0.84 and the IC50 was 67.37 µg/mL. The maximum Fe3+ reduction and Mo6+ reduction were 54.85±0.35 % and 61.13±0.21 % at 60 µg/mL concentration and the reduction concentration (RC50) were 51.59 µg/mL and   28.69 µg/mL concentration respectively.


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Leela N.K. (2008). Nutmeg and mace, In: Parthasarathy V.P., Chempaka B., Zacharias T.J. (Eds.), Chemistry of spices, CABI International, Wellington, UK, pp. 165-189.

Ozaki Y, Soedigdo S, Wattimena YR, Suganda AG. (1989). Anti-inflammatory effects of mace, aril of myristica fragrans houtt., and its active principles. Jpn J Pharmacol. 49, 155-63.

Saeki Y, Ito Y, Shibata M, Sato Y, Okuda K, Takazoe I. (1989). Antimicrobial action of natural substances on oral bacteria. Bull Tokyo Dental College, 30(3), 129-35.

Blois MS. (1958). Antioxidant determinations by the use of a stable free radical. Nature, 29, 1199-1200.

Ravishankara MN, Shrivatsava N, Padh H, Rajani M. (2002). Evaluation of antioxidant properties of root bark of hemidesmus indicus. Phytomedicine, 9, 153-60.

Yen. G C, and Chen. H Y. (1995). Antioxidant activity of various tea extracts in relation to their antimutagenicity. J. Agri. Food Chem, 43, 27-32.

Prieto. P, Pineda. M and Anguilar. M. (1999). Spectrophtometric quantisation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal. Biochem, 269, 337-341.

Kubo I., Muroi H. and Himejima M. (2002). Antimicrobial activity of green tea flavor components and their combination effects. Journal of Agric Food Chem., 4, 245-248.

Stahl E. (2005). Thin Layer Chromatography. (2nd ed.). Springer Pvt. Ltd., New Delhi, pp. 85.

Harini V, Vijayalakshmi M, Sivaraj C, and Arumugam P. (2017). Antioxidant and anticancer activities of methanol extract of melochia corchorifolia l. Int. J. Of Sci and Res., 6(1), 1310-1316.

Kedare SB & Singh RP. (2011). Genesis and development of DPPH method of antioxidant assay. J Food Sci Techol, 4, 412-22.

Gülçin İ., Oktay M., Küfrevioğlu Ö. İ., and Aslan A. (2002). Determination of antioxidant activity of lichen Cetrariaislandica (L) Ach. Journal of Ethnopharmacology, 79 (3), 325-329.

A. Y. Loo, Jain Noordin Kassim, and Darah Ibrahim. (2008). Antioxidant activity of compounds isolated from the pyroligneous acid, Rhizophora apiculata. Food Chemistry 107(3), 1151-1160.

Perumal Siddhuraju and Klaus Becker. (2007). The antioxidant and free radical scavenging activities of processed cowpea (vigna unguiculata (l.) walp.) seed extracts. Food Chemistry 101(1), 10-19.

Maher Obeidat, Mohamad Shatnawi, Mohammad Al-alawi, Enas Al-Zu`bi, Hanee Al-Dmoor, Maisa Al-Qudah, Jafar El-Qudah, and Ismael Otri. (2012). Antimicrobial activity of crude extracts of some plant leaves. Research Journal of Microbiology, 7(1), 59-67

How to Cite
S. Mahalakshmi, V. S. Sruthi, N. G. Ramesh Babu, C. Sivaraj, & P. Arumugam. (2019). Isolation and Pharmacological Activities of Curcumin from Curcuma longa L. International Journal for Research in Applied Sciences and Biotechnology, 6(5), 7-17.