Bio-removal Efficiency of Glyphosate by Using Indigenous Laccase Producing Fungi

  • Tin Myat Swe Ministry of Education
  • Win Nandar
  • Htike Htike Ei
  • Nwe Nwe Win
  • Kyi Kyi Swe
  • Thet kyaw Ko
  • Theint Theint Win Ministry of Education,
Keywords: Fungi, Glyphosate-resistant, Indigenous, Laccase, Mycoremdaition

Abstract

This study was aimed to determine the degradation potential of indigenous fungi isolated from glyphosate contaminated soil. A total number of 10 fungal strains have been isolated and screened their tolerance level against various concentrations of glyphosate (200, 400, 600, 800 and 1000 ppm).These strains were also screened for laccase activity. Only two strains 5p and 6m showed laccase activity and they are characterized, identified and selected for myco-remediation experiment. Due to its macro- and microscopic morphology of mycelia and the molecular similarity, the isolate 5p was identified as Trichoderma harzianum and the isolate 6m as Aspergillus Aiger. 1000ppm of Glyphosate disappeared rapidly in mineral salt liquid media by T.harzianum 78.1% and A.niger 87.3%. Glyphosate almost disappeared by T. harzianum 67.1 % and A. niger 67.2% in the water medium. Based on present findings, these fungal strains can be recommended as potentially effective to protect the environment from the herbicide residues.

 

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Published
2020-10-03
How to Cite
Swe, T. M., Nandar, W., Ei, H. H., Nwe Nwe Win, Kyi Kyi Swe, Thet kyaw Ko, & Win, T. T. (2020). Bio-removal Efficiency of Glyphosate by Using Indigenous Laccase Producing Fungi. International Journal for Research in Applied Sciences and Biotechnology, 7(5), 249-256. https://doi.org/10.31033/ijrasb.7.5.32