Bio-removal Efficiency of Glyphosate by Using Indigenous Laccase Producing Fungi

  • Tin Myat Swe Ministry of Education
  • Win Nandar
  • Htike Htike Ei
  • Nwe Nwe Win
  • Kyi Kyi Swe
  • Thet kyaw Ko
  • Theint Theint Win Ministry of Education,
Keywords: Fungi, Glyphosate-resistant, Indigenous, Laccase, Mycoremdaition


This study was aimed to determine the degradation potential of indigenous fungi isolated from glyphosate contaminated soil. A total number of 10 fungal strains have been isolated and screened their tolerance level against various concentrations of glyphosate (200, 400, 600, 800 and 1000 ppm).These strains were also screened for laccase activity. Only two strains 5p and 6m showed laccase activity and they are characterized, identified and selected for myco-remediation experiment. Due to its macro- and microscopic morphology of mycelia and the molecular similarity, the isolate 5p was identified as Trichoderma harzianum and the isolate 6m as Aspergillus Aiger. 1000ppm of Glyphosate disappeared rapidly in mineral salt liquid media by T.harzianum 78.1% and A.niger 87.3%. Glyphosate almost disappeared by T. harzianum 67.1 % and A. niger 67.2% in the water medium. Based on present findings, these fungal strains can be recommended as potentially effective to protect the environment from the herbicide residues.



Download data is not yet available.


Tu, M., Hurd, C. and Randall, J. M. (2001). Weed Control Methods Handbook. The Nature Conservancy, Version: April.

Marican, A., & Durán-Lara, E. F. (2018). A review on pesticide removal through different processes. Environmental Science and Pollution Research, 25(3), 2051–2064.

Zhang, J. L., & Qiao, C. L. (2002). Novel approaches for remediation of pesticide pollutants. International Journal of Environment and Pollution, 18(5), 423.

Schoefs, O., Perrier, M., & Samson, R. (2004). Estimation of contaminant depletion in unsaturated soils using a reduced-order biodegradation model and carbon dioxide measurement. Applied Microbiology and Biotechnology, 64(1), 53–61.

S., P. (2001). Feasibility of bioremediation by white-rot fungi. Applied Microbiology and Biotechnology, 57(1–2), 20–33.

Evans, C.S. and Hedger, J.N. (2001). Degradation of plant cell wall polymers. in British mycological society symposium series.

Šašek, V., Glaser, J. A., & Baveye, P. (Eds.). (2003). Why mycoremediations have not yet come into practice, In: The Utilization of Bioremediation to Reduce Soil Contamination: Problems and Solutions. Springer Netherlands. pp. 247-266.

Baldrian, P. (2006). Fungal laccases – occurrence and properties. FEMS Microbiology Reviews, 30(2), 215–242.

Piontek, K., Antorini, M., & Choinowski, T. (2002). Crystal Structure of a Laccase from the Fungus Trametes versicolor at 1.90-Å Resolution Containing a Full Complement of Coppers. Journal of Biological Chemistry, 277(40), 37663–37669.

Gianfreda, L., Xu, F., & Bollag, J.-M. (1999). Laccases: A Useful Group of Oxidoreductive Enzymes. Bioremediation Journal, 3(1), 1–26.

Liu, J., Cai, Y., Liao, X., Huang, Q., Hao, Z., Hu, M., Zhang, D., & Li, Z. (2013). Efficiency of laccase production in a 65-L air-lift reactor for potential green industrial and environmental application. Journal of Cleaner Production, 39, 154–160.

D'Souza, T.M., Boominathan, K., and Reddy, C.A. (1996). Isolation of laccase gene-specific sequences from white rot and brown rot fungi by PCR. Applied and Environmental Microbiology, 62(10), 3739-3744.

Rodríguez Couto, S., & Toca Herrera, J. L. (2006). Industrial and biotechnological applications of laccases: A review. Biotechnology Advances, 24(5), 500–513.

Telke, A. A., Kadam, A. A., Jagtap, S. S., Jadhav, J. P., & Govindwar, S. P. (2010). Biochemical characterization and potential for textile dye degradation of blue laccase from Aspergillus ochraceus NCIM-1146. Biotechnology and Bioprocess Engineering, 15(4), 696–703.

Eman, A., Abdel-Megeed, A., Suliman, A., Sadik, M. and Sholkamy, E. N. (2013). Biodegradation of glyphosate by fungal strains isolated from herbicides polluted-soils in Riyadh area. British Journal of Environmental Sciences, 1(1), 7-29.

More, S. S., P. S., R., K., P., M., S., Malini, S., & S. M., V. (2011). Isolation, Purification, and Characterization of Fungal Laccase from Pleurotus sp. Enzyme Research, 2011, 1–7.

Rifai, M.A., (1969).A revision of the genus Trichoderma. Mycological Papers, 116, 1-56.

White, T. J., Bruns, T., Lee, S., & Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal rna genes for phylogenetics. In PCR Protocols (pp. 315–322). Elsevier.

Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., & Kumar, S. (2011). MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution, 28(10), 2731–2739.

Zalat, O. A., Elsayed, M. A., Fayed, M. S., & Abd El Megid, M. K. (2013). Validation of UV Spectrophotometric and HPLC Methods for Quantitative Determination of Chlorpyrifos. International Letters of Chemistry, Physics and Astronomy, 21, 58–63.

Pawar, K. and Mali, G. (2014). Biodegradation of Quinolphos insecticide by Pseudomonas strain isolated from grape rhizosphere soils. International Journal of Current Microbiology and Applied Sciences, 3(2), 606-613.

Tazdaït, D., Salah, R., Grib, H., Abdi, N., & Mameri, N. (2018). Kinetic study on biodegradation of glyphosate with unacclimated activated sludge. International Journal of Environmental Health Research, 28(4), 448–459.

Busse, M. D., Ratcliff, A. W., Shestak, C. J., & Powers, R. F. (2001). Glyphosate toxicity and the effects of long-term vegetation control on soil microbial communities. Soil Biology and Biochemistry, 33(12–13), 1777–1789.

Santos, A., & Flores, M. (1995). Effects of glyphosate on nitrogen fixation of free-living heterotrophic bacteria. Letters in Applied Microbiology, 20(6), 349–352.

Krzyśko-Łupicka, T., & Orlik, A. (1997). The use of glyphosate as the sole source of phosphorus or carbon for the selection of soil-borne fungal strains capable to degrade this herbicide. Chemosphere, 34(12), 2601–2605.

Singh, R., (2014). Microorganism as a tool of bioremediation technology for cleaning environment: a review. Proceedings of the International Academy of Ecology and Environmental Sciences, 4(1), 1.

Ck, H. (2012). Effect of Pesticide (Chlorpyrifos) on Soil Microbial Flora and Pesticide Degradation by Strains Isolated from Contaminated Soil Hindu. Journal of Bioremediation & Biodegradation, 04(02).

Viswanath, B., Rajesh, B., Janardhan, A., Kumar, A. P., & Narasimha, G. (2014). Fungal Laccases and Their Applications in Bioremediation. Enzyme Research, 2014, 1–21.

Samuel Mwafulirwa, G.O., Placid Mpeketula and Gabriel Aboge (2017). Isolation of indigenous glyphosate degrading microbes from selected agroecological zones of Malawi. Research Journal of Recent Sciences. 6(12), 1-8.

Kamala, Th., Devi, S. I., Sharma, K. C., & Kennedy, K. (2015). Phylogeny and Taxonomical Investigation of Trichoderma spp. From Indian Region of Indo-Burma Biodiversity Hot Spot Region with Special Reference to Manipur. BioMed Research International, 2015, 1–21.

Gautam, A. and Bhadauria, R. (2012). Characterization of Aspergillus species associated with commercially stored triphala powder. African journal of Biotechnology, 11(104), 16814-16823.

A, A.-M., Mw, S., Ho, A.-S., & Hm, A. (2013). Phyto-microbial degradation of glyphosate in riyadh area. International Journal of Microbiology Research, 5(5), 458–466.

Souza, A. P. de, Ferreira, F. A., Silva, A. A. da, Cardoso, A. A., & Ruiz, H. A. (1999). Respiração microbiana do solo sob doses de glyphosate e de imazapyr. Planta Daninha, 17(3), 387–398.

How to Cite
Swe, T. M., Nandar, W., Ei, H. H., Nwe Nwe Win, Kyi Kyi Swe, Thet kyaw Ko, & Win, T. T. (2020). Bio-removal Efficiency of Glyphosate by Using Indigenous Laccase Producing Fungi. International Journal for Research in Applied Sciences and Biotechnology, 7(5), 249-256.