Effect of Diode Laser on Antibiotic Sensitivity and Biofilm Formation by Streptococcus Spp. Isolated from Dental Caries

Authors

  • Saja Ali Shareef Assistant Lecturer, Department of Medical Techniques Laboratories, Al-Farabi University College, Baghdad, IRAQ.
  • Aya Saadoon Dawood Department of Molecular and Medical Biotechnologh, College of Biotechnology, Al-Nahrain University, Baghdad, IRAQ.
  • Reem Naeem Al-Azzawi Lecturer, Department of Molecular and Medical Biotechnologh, College of Biotechnology, Al-Nahrain University, Baghdad, IRAQ.

DOI:

https://doi.org/10.31033/ijrasb.9.3.23

Keywords:

Dental caries, Streptococcus spp., Diode laser

Abstract

Streptococci from dental caries were isolated and identified during this study. The samples were collected from different Dental clinics in Baghdad. Cultural, morphological and biochemical test were used to identify all isolates, the results showed that all isolates belong to Streptococcus spp

The susceptibility of 10 isolates toward 10 of common antibiotics, was tested, results showed that several isolates of Streptococcus spp. Isolated from dental caries were resistant to most of the antibiotics used. The percentages of resistance were 100% of isolates resistant to cephalothin and cefixime, 90% of isolates resistant to ampicillin, naldixic acid and erythromycin, 70% of isolates resistant to bacitracin and tetracycline, while 100% of isolates sensitive to gentamycin, ciprofloxacin and chloramphenicol.

The susceptibility to antibiotics of two isolates after exposure to diode laser was increased toward chloramphenicol, tetracycline and naldixic acid. Two isolates showed increased susceptibility to chloramphenicol after I minute from exposure to diode laser.

Also two isolates showed increased susceptibility to naldixic acid after 3 minute from exposure to diode lases. The increasing susceptibility to tetracycline begin after 10 minute for second isolate, while first isolate showed increased susceptibility to tetracycline after 5 minute from exposure to diode laser. Also the diode laser affected on viability of bacteria that increased with increased time of exposure.

Downloads

Download data is not yet available.

References

Ahn SJ, Wen ZT, Brady LJ, Burne RA (2008) Characteristics of biofilm formation by Streptococcus mutans in the presence of saliva. Infect Immun 76(9):4259–4268.

Brady LJ, Maddocks SE, Larson MR, Forsgren N, Persson K, Deivanayagam CC, Jenkinson HF (2010) The changing faces of Streptococcus antigen I/II polypeptide family adhesins. Mol Microbiol 77(2):276–286.

Lai, Y.; Tang, V.; Aspirase, B. M.; Lau, Y. P.; Lee, H. J.; Ellen, P. R. and Cvitkovitch, G. D. (2002). A Quorum-sensing signaling system essential for genetic competence in Streptococcus mutans is involved in biofilm formation. Bacteriol. 184(10): 2699-2708.

Yamashita Y, Bowen WH, Burne RA, Kuramitsu HK. Role of the Streptococcus mutans gtf genes in caries induction in the specific-pathogen-free rat model. Infect Immun. 1993; 61:3811–3817.

Chopra, S; Chawla, H.M. (1992).Laser in chemical and biological science wiedly. Eastern limited pub co.

K. Štulík, in Encyclopedia of Analytical Science (Second Edition), 2005.

Matthew S. Brown and Craig B. Arnold Fundamentals of Laser-Material Interaction and Application to Multiscale Surface Modification, 2010.

Manni JG.(2004) Dental Applications of Advanced Lasers. April

2000 Burlington, Massachusetts: JGM Associates, Inc., 2000:2-16.

Christensen GD, Bisno AL, Simpsom WA, Beachey EH. Adherence of slime producing strains of Staphylococcus epidermidis to smooth surfaces. Infect Immun. 1982;37:318–326.

Atlas, R.M. (1995).Principle of microbiology (1st ed.) Mosby-Year bock Inc.

AL- Rassam, Y.Z. (2010). The Effect of Laser Light on Virulence Factors and Antibiotic Susceptibility of Locally Isolated Pseudomonas aeruginosa J. Appl. Sci. Res., 6(8): 1298-1302.

Zimbro, M. J.; Power, D. A.; Miller, S. M.; Wilson, G. E. and Johnsong J. A. (1998). Difico Manual, 11th edition. USA.

Yoo, S.; Kim, P.; Hwang, H.; Kim, K.; Choe, S.; Min, B. and Kook, J. (2005). Identification of non-mutans streptococci organisms in dental plaques recovering on mitis salivarius bacitracin agar medium. Microbiol. 43 (2):204-208.

Bush K, Jacoby GA, Medeiros AA. 1995. A functional classification scheme for beta-lactamases and its correlation with molecular structure. Anti-microbe Agents Chemother. 39:1211–1233.

Brown, D.F.J.; Hope, R.; Livermore, D.M.; Brick, G.;Broughton, K.; George, R.C. and R. Reynolds (2008). Non-susceptibility trends among enterococci and non-pneumococcal streptococci from bacteraemias in the UK and Ireland.

J.Antimicrob. Chemther. 62: 75–85.

Featherstone et al., 1997; Chin-Ying et al., 2004; Tepper et al; 2004.

Kim Robin Segal,1998. Diode laser irradiation system for biological tissue stimulation . Patent 5,755,752.

Karu,T.(1993) Primary and Secondary Mechanisms of Action of Monochromatic Visible and IR Radiation on Cell. pp: 99-108.

Downloads

Published

2022-06-15

How to Cite

Saja Ali Shareef, Aya Saadoon Dawood, & Reem Naeem Al-Azzawi. (2022). Effect of Diode Laser on Antibiotic Sensitivity and Biofilm Formation by Streptococcus Spp. Isolated from Dental Caries. International Journal for Research in Applied Sciences and Biotechnology, 9(3), 139–142. https://doi.org/10.31033/ijrasb.9.3.23

Issue

Vol. 9 No. 3 (2022): May Issue

Section

Articles

License

Copyright (c) 2022 International Journal for Research in Applied Sciences and Biotechnology

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.